In vitro activity of panomycocin, a novel exo-beta-1,3-glucanase isolated from Pichia anomala NCYC 434, against dermatophytes

Izgu, Fatih
Altinbay, Demet
Tureli, Akif Emre
Killer proteins that are produced and secreted into the environment by certain yeast strains are considered as promising antifungal agents. In this study, in vitro activity of Pichia anomala NCYC 434 (K5) killer protein, panomycocin, which is a 49 kDa glycoprotein with an exo-beta-1,3-glucanase activity was tested against 41 isolates of dermatophytes. Minimum inhibitory concentrations (MICs) were determined by a broth microdilution method based on the reference document M38-A of Clinical and Laboratory Standards Institute (CLSI; formerly NCCLS). For panomycocin MIC determinations two end point criteria MIC-2 (prominent growth inhibition) and MIC-0 (complete growth inhibition) were recorded. All the tested isolates (Microsporum spp. and Trichophyton spp.) were found susceptible to panomycocin. The MIC-2 values ranged from 0.25 to 2 mu g ml(-1) and MIC-0 values ranged from 1 to 8 mu g ml(-1). These results showed that panomycocin is active in vitro against fungal strains that cause superficial infections and highlighted its probable use as a topical antifungal agent.


Antifungal spectrum determination of the K5 type yeast killer protein on fungi causing spoilage in citrus fruits
Kepekçi, Remziye Aysun; İzgü, Kadri Fatih; Department of Biology (2006)
Some yeast strains under certain conditions secrete polypeptide toxins which are inhibitory to sensitive fungal cells into the medium. These yeast strains are termed as killer yeasts and their toxins are designated as killer proteins or killer toxins. Killer proteins are classified into 11 typical types (K1-K11). These toxins have different killing mechanisms on sensitive cells. Some of them hydrolyze major cell wall component, beta-1,3- glucans. As mammalian cells lack cell walls research and development o...
In vivo interaction of carcinogenic acrylamide with cytochrome p450 isozymes and phase II enzymes in rabbit liver, kidney and lung
Nuyan, Mine; Arınç, Emel; Department of Biochemistry (2008)
Acrylamide is an industrially produced chemical with known neurotoxic, reproductive toxin and carcinogenic effects. The carcinogenicity associated with acrylamide is mostly attributed to its metabolism by liver CYP2E1. However, studies investigating the effects of acrylamide on CYP2E1 enzyme are limited. In this study, it was aimed to investigate in vivo interaction of carcinogenic acrylamide on microsomal cytochrome P450 enzyme activities, and protein levels, and on cytosolic NQO1 and GST enzyme activities...
In-silico determination of Pichia pastoris signal peptides for extracellular recombinant protein production
Massahi, Aslan; Çalık, Pınar (2015-01-07)
In-silico identified novel secretory signal peptides (SPs) are required in vivo to achieve efficient transfer or to prevent other cellular proteins from interfering with the process in extracellular recombinant protein (r-protein) production. 56 endogenous and exogenous secretory SPs, have been used or having the potential to be used in Pichia pastoris for r-protein secretion, were analyzed in-silico using the softwares namely SignalP4.1, Phobius, WolfPsort0.2, ProP1.0, and NetNGlyc1.0. Among the predicted ...
Targeted disruption of homoserine dehydrogenase gene in Streptomyces clavuligerus and its effects on cephamycin C production
Çaydaşı (Koca), Ayşe; Özcengiz, Gülay; Department of Biology (2006)
The members of the genus Streptomyces are well-known for their capacity to synthesize a vast repertoire of secondary metabolites, including many useful antibiotics and proteins. Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics such as cephamycin C and the potent β-lactamase inhibitor clavulanic acid. The aspartate pathway of S. clavuligerus is an important primary metabolic pathway providing substrates for β-lactam synthesis. This pathway uses L-aspartic acid as the ...
Investigation of cytocidal effect of K5 type yeast killer protein on sensitive microbial cells
Sertkaya, Abdullah; İzgü, Kadri Fatih; Department of Biology (2005)
Some yeasts secrete polypeptide toxins, which are lethal to other sensitive yeast cells, gram-positive pathogenic bacteria and pathogenic fungi. Therefore these are designated as killer toxins. Killer toxins are suggested as potent antimicrobial agents especially for the protection of fermentation process against contaminating yeasts, biological control of undesirable yeasts in the preservation of foods. Moreover they are promising antimicrobial agents in the medical field; due to immune system suppressing ...
Citation Formats
F. Izgu, D. Altinbay, and A. E. Tureli, “In vitro activity of panomycocin, a novel exo-beta-1,3-glucanase isolated from Pichia anomala NCYC 434, against dermatophytes,” MYCOSES, pp. 31–34, 2007, Accessed: 00, 2020. [Online]. Available: