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Affinity interaction of hydroxypyruvate reductase from Methylophilus spp. with Cibacron blue F3GA-derived poly(HEMA EGDMA) microspheres: partial purification and characterization
Date
1999-06-01
Author
Arica, MY
Halicigil, C
Alaeddinoglu, G
Denizli, A
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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A methylotrophic hydroxypyruvate reductase was partially purified and characterized from Methylophilus spp. using the biomimetic dye, Cibacron Blue F3FA attached to poly(HEMA-EGDMA) microspheres. The absorption capacities of the dye-affinity microspheres were determined by changing pH and the concentration of the proteins in the adsorption medium. Hydroxypyruvate reductase was desorbed from the dye-affinity support specifically with 2 mM NADH solution. The enzyme was purified 10.4-fold with 47% yield. The molecular mass and subunit molecular mass of the enzyme was estimated to be 75 kDa and 37 kDa on the basis of its mobility in polyacrylamide and SDS-polyacrylamide gels, respectively. This suggested a homogeneous dimer structure. The optimal pH was between 5.0 and 7.0, and the maximum enzyme activity was obtained at 50 degrees C. The K-m values of hydroxpyruvate reductase were 0.222 mM for hydroxpyruvate and 0.067 mM for NADH.
Subject Keywords
Hydroxypyruvate reductase
,
NADH dependent enzyme
,
Methylophilus spp.
,
Affinity adsorption
,
Poly(HEMA-EGDMA) microspheres
,
Cibacron Blue F3GA
URI
https://hdl.handle.net/11511/67723
Journal
PROCESS BIOCHEMISTRY
DOI
https://doi.org/10.1016/s0032-9592(98)00104-6
Collections
Department of Biology, Article