Quantitative Analysis of MAP-Mediated Regulation of Microtubule Dynamic Instability In Vitro—Focus on Tau

Date

2010-05-01

Author

Kiriş, Erkan
Feinstein, Stuart C.

Metadata

Show full item record

Item Usage Stats

221
views

0
downloads

The regulation of microtubule growing and shortening dynamics is essential for proper cell function and viability, and microtubule-associated proteins (MAPs) such as the neural protein tau are critical regulators of these dynamic processes. Further, we and our colleagues have proposed that misregulation of microtubule dynamics may contribute to tau-mediated neuronal cell death and dementia in Alzheimer's and related diseases. In the first part of this chapter, we present a general background on microtubule dynamics and then focus in on tau. We review the literature on the roles of tau in normal neuronal cell biology, the tau structure-function relationship, regulatory mechanisms influencing tau action, and pathological tau action, including normal and aberrant regulation of microtubule dynamics. In the second part of this chapter, we present detailed protocols for various in vitro procedures often used in studying tau-mediated regulation of microtubule dynamics, including purification and characterization of necessary reagents, microtubule assembly assays, and microtubule dynamics assays. Importantly, these assays are readily adaptable to examine other regulators of microtubule dynamics besides tau. In the final analysis, in vitro analyses of MAP-mediated regulation of microtubule dynamics will provide extremely valuable insights into our understanding of normal and pathological cell biology.

URI

https://www.sciencedirect.com/bookseries/methods-in-cell-biology/vol/95/suppl/C
https://hdl.handle.net/11511/71063

Relation

Methods In Cell Bıology

Collections

Department of Biology, Book / Book chapter

Suggestions

OpenMETU
Core

Quantitative Analysis of MAP-Mediated Regulation of Microtubule Dynamic Instability In Vitro: Focus on Tau Kiriş, Erkan; Feinstein, Stuart C (Elsevier, 2010-01-01) The regulation of microtubule growing and shortening dynamics is essential for proper cell function and viability, and microtubule-associated proteins (MAPs) such as the neural protein tau are critical regulators of these dynamic processes. Further, we and our colleagues have proposed that misregulation of microtubule dynamics may contribute to tau-mediated neuronal cell death and dementia in Alzheimer’s and related diseases. In the first part of this chapter, we present a general background on microtubule ...
Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters SHIN, Ilchung; RAY, Judhajeet; Gupta, Vinayak; İlgü, Müslüm; Beasley, Jonathan; BENDICKSON, Lee; MEHANOVIC, Samir; Kraus, George A.; Nilsen-Hamilton, Marit (Oxford University Press (OUP), 2014-01-01) We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that can be expressed from a promoter of choice. For imaging, the cells are incubated with their ligands that are separately conjugated with one of the FRET pair, Cy3 and Cy5. The IMAGEtags were expressed in yeast from ...
Inference of Gene Regulatory Networks Via Multiple Data Sources and a Recommendation Method Ozsoy, Makbule Gulcin; Polat, Faruk; Alhajj, Reda (2015-11-12) Gene regulatory networks (GRNs) are composed of biological components, including genes, proteins and metabolites, and their interactions. In general, computational methods are used to infer the connections among these components. However, computational methods should take into account the general features of the GRNs, which are sparseness, scale-free topology, modularity and structure of the inferred networks. In this work, observing the common aspects between recommendation systems and GRNs, we decided to ...
Analysis of protein-protein interaction networks using random walks Can, Tolga; Singh, Ambuj K. (2005-08-21) Genome wide protein networks have become reality in recent years due to high throughput methods for detecting protein interactions. Recent studies show that a networked representation of proteins provides a more accurate model of biological systems and processes compared to conventional pair-wise analyses. Complementary to the availability of protein networks, various graph analysis techniques have been proposed to mine these networks for pathway discovery, function assignment, and prediction of complex mem...
Inference of large-scale networks via statistical approaches Ayyıldız Demirci, Ezgi; Purutçuoğlu Gazi, Vilda; Department of Statistics (2019) In system biology, the interactions between components such as genes, proteins, can be represented by a network. To understand the molecular mechanism of complex biological systems, construction of their networks plays a crucial role. However, estimation of these networks is a challenging problem because of their high dimensional and sparse structures. The Gaussian graphical model (GGM) is widely used approach to construct the undirected networks. GGM define the interactions between species by using the con...

Citation Formats

E. Kiriş and S. C. Feinstein, Quantitative Analysis of MAP-Mediated Regulation of Microtubule Dynamic Instability In Vitro—Focus on Tau. 2010, p. 503.