Tracing microtubules in live cell images

Date

2007-06-15

Author

Sargin, Mehmet Emre
Altinok, Alphan
Kiriş, Erkan
Feinstein, Stuart C
Wilson, Leslie
Rose, Kenneth
Manjunath, B S

Metadata

Show full item record

Item Usage Stats

98
views

0
downloads

Microtubule (MT) dynamics are traditionally analyzed from time lapse images by manual techniques that are laborious, approximate and often limited. Recently, computer vision techniques have been applied to the problem of automated tracking of MTs in live cell images. Aside of very low signal to noise ratios, live cell images of MTs exhibit severe clutter for accurate tracing of MT body. Moreover, intersecting and overlapping MT regions appear brighter due to additive fluorescence. In this paper, we present a MT body tracing algorithm that addresses the clutter without imposing directional constraints. We show that MT dynamics can be quantified with enhanced precision, and novel measurements that are beyond manual feasibility, can be obtained accurately. We demonstrate our results on actual images of MTs obtained by live cell fluorescence microscopy.

Subject Keywords

Biomedical image processing, Image line pattern analysis, Object detection

URI

https://hdl.handle.net/11511/71941
http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=16&SID=E15OHmhMPnIIbI3YXrp&page=1&doc=1

Conference Name

4th IEEE International Symposium on Biomedical Imaging (April 12-15, 2007)

Collections

Department of Biology, Conference / Seminar

Suggestions

OpenMETU
Core

Alignment of uncalibrated images for multi-view classification Arık, Sercan Ömer; Vural, Elif; Frossard, Pascal (2011-12-29) Efficient solutions for the classification of multi-view images can be built on graph-based algorithms when little information is known about the scene or cameras. Such methods typically require a pairwise similarity measure between images, where a common choice is the Euclidean distance. However, the accuracy of the Euclidean distance as a similarity measure is restricted to cases where images are captured from nearby viewpoints. In settings with large transformations and viewpoint changes, alignment of im...
Ghost Removal in High Dynamic Range Images Khan, Erum Arif; Akyüz, Ahmet Oğuz; Reinhard, Erik (2006-10-11) High dynamic range images may be created by capturing multiple images of a scene with varying exposures. Images created in this manner are prone to ghosting artifacts, which appear if there is movement in the scene at the time of capture. This paper describes a novel approach to removing ghosting artifacts from high dynamic range images, without the need for explicit object detection and motion estimation. Weights are computed iteratively and then applied to pixels to determine their contribution to the fin...
An Application of inpainting-based still image reanimation Böncü, Ece Selin; Akar, Gözde; Department of Electrical and Electronics Engineering (2017) A single-input system that converts still paintings into short video sequences is developed. The system is composed of four separate blocks that are Digital Matting, Inpainting, Motion Modelling & Synthesis and Rendering Blocks. Within the scope of this thesis, the performance analysis of all are realized and the input images are restricted to paintings so as not to cope with the difficulty of achieving photorealism that accompanies the usage of photographs. The input image is partitioned into object-backgr...
Image fusion for improving spatial resolution of multispectral satellite images Ünlüsoy, Deniz; Süzen, Mehmet Lütfi; Department of Geological Engineering (2013) In this study, four different image fusion techniques have been applied to high spectral and low spatial resolution satellite images with high spatial and low spectral resolution images to obtain fused images with increased spatial resolution, while preserving spectral information as much as possible. These techniques are intensity-hue-saturation (IHS) transform, principle component analysis (PCA), Brovey transform (BT), and Wavelet transform (WT) image fusion. Images used in the study belong to Çankırı reg...
Matching of straight line segments from aerial stereo images of urban areas Ok, Ali Ozgun; Wegner, Jan Dirk; Heipke, Christian; Rottensteiner, Franz; Soergel, Uwe; Toprak, Vedat (2012-11-01) Reliable extraction of corresponding straight lines in overlapping images can be used for different purposes such as 3D object extraction, image registration, automated triangulation, etc. In this study, a new approach for the matching of straight line features from stereo aerial images is presented. Initial correspondences between stereo images are generated using a pair-wise stereo matching approach, which involves a total of seven relational constraints. The final straight line correspondences between th...

Citation Formats

M. E. Sargin et al., “Tracing microtubules in live cell images,” presented at the 4th IEEE International Symposium on Biomedical Imaging (April 12-15, 2007), Arlington, VA, 2007, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/71941.