Proteomics of Stress Response in Microbes: A Brief Overview



Proteomics of phanerochaete chrysosporium under heavy metal stress
Yıldırım, Volkan; Özcengiz, Gülay; Department of Biology (2014)
In this study, time-dependent heavy metal response of the white rot fungus P. chrysosporium was analyzed by using 2D-PAGE-MS for soluble cytosolic fraction and GeLC-MS approach for membrane enriched fraction. After the 2D-PAGE-MS analysis, a total of 123 protein spots were detected as differentially expressed for Cu-exposed samples and 89 of them were identifed. Further analysis revealed that the 89 protein spots are the products of the 58 distinct ORFs. Overall, strongly up-regulated proteins included (i) ...
Enzymic activity of the K5-type yeast killer toxin and its characterization
Izgu, F; Altinbay, D; Sertkaya, A (Informa UK Limited, 2005-11-01)
K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for lami...
Enzymatic extraction of activated sludge extracellular polymers and implications on bioflocculation
Sesay, Ml; Özcengiz, Gülay; Sanin, Faika Dilek (Elsevier BV, 2006-04-01)
This study examines enzyme hydrolysis, a mild, effective, but a rarely used method of extracellular polymer extraction, in removing polymers from mixed culture activated sludge flocs. Two carbohydrate specific enzymes (a-amylase and cellulase) and a protein specific enzyme (proteinase) are used during the study. First, the kinetic aspect is investigated, then enzyme dose optimization is carried out on laboratory grown activated sludge samples cultured at solids retention times (SRT) of 4 and 20 days. A more...
Protease activities of thermophilic microorganisms isolated from hot springs in Turkey: detection of alkaline serine protease gene sequences by polymerase chain reaction(PCR)
Erdem, Bilge; Kocabıyık, Semra; Department of Biology (1999)
Protease secretion capacity and perforce analysis of recombinant Bacillus species
Çalık, Pınar; Ozdamar, TH (2000-10-08)
Recombinant Bacillus species carrying subC gene encoding serine alkaline protease (SAP) enzyme were developed in order to increase the yield and selectivity in the bioprocess for SAP production. subC gene was amplified from the chromosomal DNA of the wild-type Bacillus licheniformis by using PCR technology; thereafter, subC gene was first cloned into the pRS316 E. coli yeast shuttle plasmid, then sub-cloned into the pHV1431 E. coli-Bacillus shuttle vector, and transferred to the host Bacillus species, i.e. ...
Citation Formats
G. Özcengiz, “Proteomics of Stress Response in Microbes: A Brief Overview,” 2008, Accessed: 00, 2021. [Online]. Available: