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Enzymic activity of the K5-type yeast killer toxin and its characterization
Date
2005-11-01
Author
Izgu, F
Altinbay, D
Sertkaya, A
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K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for laminarin hydrolysis were 0.25mg/ml and 370 mu mol/min/mg. The toxin exerted its cytocidal effect after 2 h contact with the target cells. Production of the toxin by the cells was induced only when they were grown in culture media rich in beta-glucan sources, and the addition of glucose increased the specific production rate. The enzymic activity of the toxin was fully inhibited by Hg+2, but increased with some other metal ions, most of all by Pb+2.
Subject Keywords
Biotechnology
,
Organic Chemistry
,
Analytical Chemistry
,
Applied Microbiology and Biotechnology
,
Biochemistry
,
Molecular Biology
,
General Medicine
URI
https://hdl.handle.net/11511/66737
Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
DOI
https://doi.org/10.1271/bbb.69.2200
Collections
Department of Biology, Article
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Pichia anomala NCYC 432 secretes a killer toxin which is inhibitory to a variety of yeasts including pathogenic Candida spp. The killer toxin in the culture supernatant was concentrated by ultratiltration and purified to homogenity by two successive gel filtration chromatographies with a TSK G2000SW column. Biochemical characterization of the toxin showed that it is a glycosylated protein with a molecular mass of 47 kDa and pI values of 3.4 and 3.7. The toxin showed high stability at pH values between 3 and...
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F. Izgu, D. Altinbay, and A. Sertkaya, “Enzymic activity of the K5-type yeast killer toxin and its characterization,”
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
, pp. 2200–2206, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66737.