Development of manganese-doped hydroxyapatite incorporated PCL electrospun 3D scaffolds coated with gelatin for bone tissue engineering

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2023-1-27

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Samiei, Alaleh

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Combination of polymers and bioceramics has increased their importance in bone tissue engineering (BTE) to treat various defects. Within this frame, in this thesis, it is aimed to develop a 3D gelatin-coated PCL scaffold combined with Mn-doped hydroxyapatite (HA) in order to investigate the effect of the doping element, i.e., the manganese (Mn) ion, on the structural and biological properties of the composite scaffold. Pure and Mn-doped HAs were synthesized using microwave irradiation, and the samples were sintered at 800, 900, and 1000°C. Following the evaluation of the microstructural and biological results, HA and Mn-doped HA samples sintered at 900°C were chosen for scaffold fabrications and analysis. PCL scaffold was initially constructed by electrospinning method, and gelatin-coating was done by the glutaraldehyde (GTA) cross-linker around the primary PCL scaffold. Final gelatin-coated PCL scaffold was synthesized at 10%, 15%, and 16% (w/v) PCL amounts. After the optimization, 15% (w/v) PCL coated with gelatin was modified further by adding HA and Mn-HA at two different concentrations (2.5 wt.% and 5 wt.%). Final PCL/gelatin/HA and PCL/gelatin/Mn-HA were characterized by in-vitro degradation, bioactivity, and direct cytotoxicity tests. It was concluded that PCL/gelatin with 5mol% Mn-HA had better biological properties and characteristics for BTE applications.

Subject Keywords

Bone Tissue Engineering, PCL/Gelatin scaffold, Manganese-doped Hydroxyapatite, Electrospinning, GTA Crosslinking

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https://hdl.handle.net/11511/102560

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

A. Samiei, “Development of manganese-doped hydroxyapatite incorporated PCL electrospun 3D scaffolds coated with gelatin for bone tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2023.