Asymmetric bi layer composite membrane containing Boron modified nano bioactive glass for guided bone regeneration

2015-09-16
Moonesi Reza, Rad
Atila, Deniz Hazal
Keskin, Dilek
Evis, Zafer
Tezcaner, Ayşen
Barrier membranes only allow the osteoprogenitor cells to populate in the bony defect area and prevent migration of the unfavorable cells. In this study, we designed a novel bilayered membrane composed of one layer from cellulose acetate prepared by the solvent casting method and the second layer from cellulose acetate/gelatin/boron-modified bioactive glass nanofibers prepared by the electrospinning method. Boron-modified bioactive glass particles with mole percentages of (51% SiO2, 38% CaO, 4% P2O5, 7% B2O3) were synthesized and characterized. The obtained material was amorphous and had a particle size distribution in the range of of 30-60 nm. The top surface of membrane composed of cellulose acetate was hydrophobic and had smooth non-porous structure. We prepared the bi-layered structure by incorporating the nano bioactive glass particles which generated bioactive glass gradient along the material thickness. Water-contact angles and tensile strength measurements, in vitro biomineralization test in the simulated body fluid (SBF) and in vitro biocompatibility test of the membranes using the mesenchymal stem cells and Saos-2 cell line are under investigation.

Suggestions

Immunotherapeutic applications of CpG-containing oligodeoxynucleotides
Klinman, DM; Ishii, KJ; Gürsel, Mayda; Gursel, I; Takeshita, S; Takeshita, F (2000-06-01)
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate the mammalian immune system to mount a rapid innate immune response. This response is characterized by the production of polyreactive IgM, immunomodulatory cytokines and chemokines. CpG ODN directly stimulate lymphocytes, natural killer cells and professional antigen-presenting cells (such as macrophages and dendritic cells). Owing to the strength and nature of this stimulation, CpG ODN are being harnessed f...
Anodized 20 nm diameter nanotubular titanium for improved bladder stent applications
Alpaslan, Ece; Ercan, Batur; Webster, Thomas J. (Informa UK Limited, 2011-01-01)
Materials currently used for bladder applications often suffer from incomplete coverage by urothelial cells (cells that line the interior of the bladder and ureter) which leads to the continuous exposure of the underlying materials aggravating an immune response. In particular, a ureteral (or sometimes called an ureteric or bladder) stent is a thin tube inserted into the ureter to prevent or treat obstruction of urine flow from the kidney. The main complications with ureteral stents are infection and blocka...
Micropatterned Surfaces Expose the Coupling between Actin Cytoskeleton-Lamin/Nesprin and Nuclear Deformability of Breast Cancer Cells with Different Malignancies.
Antmen Altunsoy, Ezgi; Hasirci, Vasif (2021-01-01)
Mechanotransduction proteins transfer mechanical stimuli through nucleo‐cytoskeletal coupling and affect the nuclear morphology of cancer cells. However, the contribution of actin filament integrity has never been studied directly. It is hypothesized that differences in nuclear deformability of cancer cells are influenced by the integrity of actin filaments. In this study, transparent micropatterned surfaces as simple tools to screen cytoskeletal and nuclear distortions are presented. Surfaces decorated wit...
Sequential BMP-2/BMP-7 delivery from polyester nanocapsules
Yilgor, P.; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2010-05-01)
The aim of this study was to develop a nanosized, controlled growth factor release system to incorporate into tissue engineering scaffolds and thus activate the cells seeded in the scaffold. Nanocapsules of poly(lactic acid-co-glycolic acid) (PLGA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) were loaded with the bone morphogenetic proteins BMP-2 and BMP-7, respectively, and with bovine serum albumin (BSA), the model protein. BSA-loading efficiency and release kinetics were used to determine the ...
Immunotherapeutic applications of CpG ODN
Gürsel, Mayda (2006-06-01)
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate the mammalian immune system to mount a rapid innate immune response. This response is characterized by the production of polyreactive IgM, immunomodulatory cytokines and chemokines. CpG ODN directly stimulate lymphocytes, natural killer cells and professional antigen-presenting cells (such as macrophages and dendritic cells). Owing to the strength and nature of this stimulation, CpG ODN are being harnessed f...
Citation Formats
R. Moonesi Reza, D. H. Atila, D. Keskin, Z. Evis, and A. Tezcaner, “Asymmetric bi layer composite membrane containing Boron modified nano bioactive glass for guided bone regeneration,” Orlando, USA, 2015, vol. 4, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/83108.