Sequential BMP-2/BMP-7 delivery from polyester nanocapsules

The aim of this study was to develop a nanosized, controlled growth factor release system to incorporate into tissue engineering scaffolds and thus activate the cells seeded in the scaffold. Nanocapsules of poly(lactic acid-co-glycolic acid) (PLGA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) were loaded with the bone morphogenetic proteins BMP-2 and BMP-7, respectively, and with bovine serum albumin (BSA), the model protein. BSA-loading efficiency and release kinetics were used to determine the most appropriate nanocapsule pair to achieve the delivery of growth factors in a sequential manner, as occurs in natural processes. BSA-encapsulation efficiency was highest when the polymer concentration used in the preparation of PLGA and PHBV nanocapsules was 10% (w/v) (84.75% and 16.72%, respectively). Release of BSA was faster from PEGA than it was from PHBV. Based on the encapsulation efficiency and release data, 10% PLGA and 1.0% PHBV nanocapsules were chosen to provide the early BMP-2 and later BMP-7 release, respectively. Simultaneous, sequential delivery and individual release of the BMPs were studied for 7, 14, and 21 days, using rat bone marrow mesenchymal stem cells. Individual BMP-2 release suppressed cell proliferation while providing higher alkaline phosphatase activity with respect to BMP-7. The sequential delivery of BMP-2 and BMP-7 provided slightly lower proliferation than did simultaneous delivery, but the highest alkaline phosphatase activity of all indicated a synergistic effect on the osteogenic differentiation of mesenchymal stem cells caused by the use of the two growth factors in a sequential fashion. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 528-536, 2010


Incorporation of a sequential BMP-2/BMP-7 delivery system into chitosan-based scaffolds for bone tissue engineering
Yilgor, Pinar; Tuzlakoglu, Kadriye; Reis, Rui L.; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2009-07-01)
The aim of this study was to develop a 3-D construct carrying an inherent sequential growth factor delivery system. Poly(lactic acid-co-glycolic acid) (PLGA) nanocapsules loaded with bone morphogenetic protein BMP-2 and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanocapsules loaded with BIVIP-7 made the early release of BMP-2 and longer term release of BMP-7 possible. 3-D fiber mesh scaffolds were prepared from chitosan and from chitosan-PEO by wet spinning. Chitosan of 4% concentration in 2% aceti...
Sequential growth factor delivery from complexed microspheres for bone tissue engineering
Basmanav, F. Buket; KÖSE, GAMZE; Hasırcı, Vasıf Nejat (2008-11-01)
Aim of the study was to design a 3D tissue-engineering scaffold capable of sequentially delivering two bone morphogenetic proteins (BMP). The novel delivery system consisted of microspheres of polyelectrolyte complexes of poly(4-vinyl pyridine) (P4VN) and alginic acid loaded with the growth factors BMP-2 and BMP-7 which themselves were loaded into the scaffolds constructed of PLGA. Microspheres carrying the growth factors were prepared using polyelectrolyte solutions with different concentrations (4-10%) to...
A biomimetic growth factor delivery strategy for enhanced regeneration of iliac crest defects
Huri, Pinar Yilgor; Huri, Gazi; Yasar, Umit; UÇAR , YURDANUR; DİKMEN, NURTEN; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2013-06-01)
The importance of provision of growth factors in the engineering of tissues has long been shown to control the behavior of the cells within the construct and several approaches were applied toward this end. In nature, more than one type of growth factor is known to be effective during the healing of tissue defects and their peak concentrations are not always simultaneous. One of the most recent strategies includes the delivery of a combination of growth factors with the dose and timing to mimic the natural ...
Modified Hantzsch Reaction in the Presence of Chiral Organic Catalysts
Magerramov, A. M.; Guseinov, E. Z.; Akhmedov, I. M.; Tanyeli, Dj.; Kurbanova, M. M. (2016-05-01)
Modified Hantzsch reaction in three- and four-component system in the presence of new chiral organic catalysts was investigated and the advantages of this reaction in three- component system compared to four-component one were demonstrated, the influence of catalysts on the dynamics and stereochemistry of the reaction was elucidated. The enantiomeric excess of the main reaction product was evaluated with HPLC, its structure was proved by X-ray diffraction analysis.
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Kale, Emre H.; Mumcuoğlu, Ünal Erkan; HAMCAN, Salih (2012-12-01)
The aim of this study was to develop automatic image segmentation methods to segment human facial tissue which contains very thin anatomic structures. The segmentation output can be used to construct a more realistic human face model for a variety of purposes like surgery planning, patient specific prosthesis design and facial expression simulation. Segmentation methods developed were based on Bayesian and Level Set frameworks, which were applied on three image types: magnetic resonance imaging (MRI), compu...
Citation Formats
P. Yilgor, N. Hasırcı, and V. N. Hasırcı, “Sequential BMP-2/BMP-7 delivery from polyester nanocapsules,” JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, pp. 528–536, 2010, Accessed: 00, 2020. [Online]. Available: