Targeted Drug Delivery via Chitosan-Coated Magnetic Nanoparticles

2017-01-01
Unsoy, Gözde
Gündüz, Ufuk
The devastating effects of chemotherapeutic agents have been observed on healthy cells as well as tumor cells because they are nonspecifically distributed all over the body. This treatment results in hazardous side effects and excessive toxicity. Targeted drug delivery has emerged to overcome the lack of specificity of conventional chemotherapy. Nanoparticles used for drug targeting are promising to circumvent these challenges, by enabling the localization of high drug amounts at the site of disease. When drugs are conjugated on nanoparticles, it is possible to increase their half-life while decreasing the side effects and improving therapeutic efficacy. Several therapeutic nanoparticles that selectively bind and target cancer cells have been approved for clinical use (Davis, M.E., Zuckerman, J.E., Choi, C.H.J., Seligson, D., Tolcher, A., Alabi, C.A., et al., 2010. Evidence ofRNAi in humans from systemically administered siRNA via targeted nanoparticles. Nature 464 (7291), 1067–1070.; Hrkach, J., Von Hoff, D., Ali, M.M., Andrianova, E., Auer, J., Campbell, T., et al., 2012. Preclinical developmentand clinical translation of a PSMA-targeted docetaxel nanoparticle with a differentiated pharmacologicalprofile. Sci. Transl. Med. 4 (128), 128ra39-128ra39; Tabernero, J., Shapiro, G.I., LoRusso, P.M., Cervantes, A., Schwartz, G.K., Weiss, G.J., et al., 2013. Firstin-humans trial of an RNA interference therapeutic targeting VEGF and KSP in cancer patients with liverinvolvement. Cancer Discov. 3 (4), 406–417). Surface modifications of nanoparticles with organic polymers enable the stabilization of nanoparticles, reduce agglomeration, provide functional groups for further alterations, furnish internal cavities for loading of therapeutics, and prevent immediate uptake of drug-loaded nanoparticles by the reticuloendothelial system. Polymer-coated magnetic nanoparticles, characterized by high surface to volume ratios, are excellent scaffolds for loading targeting moieties, permeation enhancers, imaging tags, and drugs, simultaneously providing diagnostic and therapeutic capabilities. Multifunctionality of nanoparticle constructs can facilitate combination therapies. Nanoparticles can be routed to the tumor tissue via passive or active targeting. Nanotechnology has the potential to change cancer diagnosis and therapy fundamentally, which may bring new insights to the chemotherapy.

Suggestions

Highly-sensitive and fast detection of human telomeric G-Quadruplex DNA based on a hemin-conjugated fluorescent metal-organic framework platform
Javan Kouzegaran, Vahid; Farhadi, Khalil; Forough, Mehrdad; Bahram, Morteza; Persil Çetinkol, Özgül (Elsevier BV, 2021-04-15)
© 2021 Elsevier B.V.The formation of G-quadruplex (G4) structures in Human telomeric DNA (H-Telo) has been demonstrated to inhibit the activity of telomerase enzyme that is associated with the proliferation of many cancer cells. Accordingly, G-quadruplex structures have become one of the well-established targets in anticancer therapeutic strategies. And, the development of simple and selective detection platforms for G4 structures has become a significant focus of research in recent years. In this study, a ...
Label-free enrichment of MCF7 breast cancer cells from leukocytes using continuous flow dielectrophoresis
Arslan, Zeynep Caglayan; Yalcin, Yagmur Demircan; Külah, Haluk (2022-04-01)
Circulating tumor cells (CTCs) present in the bloodstream are strongly linked to the invasive behavior of cancer; therefore, their detection holds great significance for monitoring disease progression. Currently available CTC isolation tools are often based on tumor-specific antigen or cell size approaches. However, these techniques are limited due to the lack of a unique and universal marker for CTCs, and the overlapping size between CTCs and regular blood cells. Dielectrophoresis (DEP), governed by the in...
Capture of rare circulating tumor cells from blood on bio-activated oxide surface inside microfluidic channels
Ateş, Hatice Ceren; Külah, Haluk; Özgür, Ebru; Department of Micro and Nanotechnology (2018)
Isolation and characterization of circulating tumor cells (CTCs) have important clinical significance in terms of prognosis and early detection of response to treatment. Moreover, downstream characterization of CTCs may help better patient stratification and therapy guidance. However, CTCs are extremely rare (~10 CTCs/1010 peripheral blood cells) and highly sensitive, and specific technology is required for their isolation. Rapidly developing microfluidic technologies offer variety of advantages in rare cel...
Targeted delivery of CPG-oligodeoxynucleotide to breast cancer cells by poly-amidoamine dendrimer-modified magnetic nanoparticles
Taghavi Pourianazar, Negar; Gündüz, Ufuk; Gündüz, Güngör; Department of Biotechnology (2016)
One major application of nanotechnology in cancer treatment involves designing nanoparticles to deliver drugs, oligonucleotides, and genes to cancer cells. Nanoparticles should be engineered so that they could target and destroy tumor cells with minimal damage to healthy tissues. This research aims to develop an appropriate and efficient nanocarrier, having the ability of interacting with and delivering CpG-oligodeoxynucleotides (CpG-ODNs) to tumor cells. CpG-ODNs activate Toll-like receptor 9 (TLR9), which...
Investigation of docetaxel and doxorubicin resistance in mcf-7 breast carcinoma cell line
Darcansoy İşeri, Özlem; Gündüz, Ufuk; Department of Biotechnology (2009)
Multidrug resistance phenotype of tumor cells describes resistance to wide range of structurally unrelated anticancer agents and is a serious limitation to effective chemotherapy. It is a multifactor yet not fully elucidated phenomenon by the involvement of diverse cellular pathways. Aim of this study was to investigate the resistance mechanisms developed against docetaxel and doxorubicin that are widely used in the treatment of breast cancer in model cell line MCF-7. Resistant sublines were developed by ap...
Citation Formats
G. Unsoy and U. Gündüz, Targeted Drug Delivery via Chitosan-Coated Magnetic Nanoparticles. 2017, p. 864.