Proteomic Analysis of a hom-Disrupted, Cephamycin C Overproducing Streptomyces clavuligerus

Date

2021-01-01

Author

Unsaldi, Eser
KURT KIZILDOĞAN, ASLIHAN
ÖZCAN, SERVET
Becher, Dorte
Voigt, Birgit
Aktas, Caner
Özcengiz, Gülay

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

116
views

0
downloads

Background: Streptomyces clavuligerus is prolific producer of cephamycin C, a medically important antibiotic. In our former study, cephamycin C titer was 2-fold improved by disrupting homoserine dehydrogenase (hom) gene of aspartate pahway in Streptomyces clavuligerus NR-RL 3585.

URI

https://hdl.handle.net/11511/90388

Journal

PROTEIN AND PEPTIDE LETTERS

DOI

https://doi.org/10.2174/0929866527666200723163655

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Quantitative expression analysis of the genes potentially involved in clavulanic acid overproduction in streptomyces clavuligerus Aktaş, Caner; Özcengiz, Gülay; Department of Molecular Biology and Genetics (2018) Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics, including cephamycin C (CC) and the potent β-lactamase inhibitor clavulanic acid. (CA). We have already undertaken an extensive comparative proteomic analysis by using both 2-DE-MALDI-MS and GeLC-MS approaches between an industrial CA overproducer, namely DEPA and the reference strain NRRL3585. In this context, we documented several differentially expressed (over- and under-represented) proteins accounting for high CA...
Genome wide evolutionary analyses reveal serotype specific patterns of positive selection in selected Salmonella serotypes Soyer, Yeşim; Rodriguez-Rivera, Lorraine D.; Sun, Qi; Wiedmann, Martin (Springer Science and Business Media LLC, 2009-11-14) Background: The bacterium Salmonella enterica includes a diversity of serotypes that cause disease in humans and different animal species. Some Salmonella serotypes show a broad host range, some are host restricted and exclusively associated with one particular host, and some are associated with one particular host species, but able to cause disease in other host species and are thus considered "host adapted". Five Salmonella genome sequences, representing a broad host range serotype (Typhimurium), two host...
Comparative Transcriptome Analyses of a Standard Strain and Clavulanic Acid Overproducing Industrial Strain of S. clavuligerus Çelik, Gözde; Özcengiz, Gülay; Son, Çağdaş Devrim; Department of Biotechnology (2021-9) Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics, including cephamycin C (CC) and potent β-lactamase inhibitor clavulanic acid (CA). In this study, comparative transcriptome analysis between an industrial stain, namely DEPA (CA overproducer strain which was developed by several rounds of classical random mutagenesis), and a reference strain NRLL3585 was performed by using the genome-wide RNA-seq technique. Our aim was to understand and improve the knowledge about the...
Isoniazid Linked to Sulfonate Esters via Hydrazone Functionality: Design, Synthesis, and Evaluation of Antitubercular Activity KOÇAK ASLAN, EBRU; HAN, MUHAMMED İHSAN; Krishna, Vagolu Siva; Tamhaev, Rasoul; Dengiz, Çağatay; DOĞAN, ŞENGÜL DİLEM; Lherbet, Christian; Mourey, Lionel; Tonjum, Tone; GÜNDÜZ, MİYASE GÖZDE (2022-10-01) Isoniazid (INH) is one of the key molecules employed in the treatment of tuberculosis (TB), the most deadly infectious disease worldwide. However, the efficacy of this cornerstone drug has seriously decreased due to emerging INH-resistant strains of Mycobacterium tuberculosis (Mtb). In the present study, we aimed to chemically tailor INH to overcome this resistance. We obtained thirteen novel compounds by linking INH to in-house synthesized sulfonate esters via a hydrazone bridge (SIH1-SIH13). Following str...
Genomic characterization of pseudomonas aeruginosa clinical isolates by pulsed field gel electrophoresis method Alipour Ghorbani, Nader; Yıldız, Fatih; Durmaz, Rıza; Department of Biotechnology (2014) Pseudomonas aeruginosa is a common cause of nosocomial infections, particularly in intensive care units (ICUs), bronchoscopy, oncology, and urology. The aim of this study was to characterize P. aeruginosa clinical isolates clonally relatedness by pulsed-field gel electrophoresis (PFGE) typing and it’s cut off value determination. We did retrospective study and analyzed genotypically a collection of 58 clinical isolates recovered during the period 2006–2011 from MESA private hospital microbiology department’...

Citation Formats

E. Unsaldi et al., “Proteomic Analysis of a hom-Disrupted, Cephamycin C Overproducing Streptomyces clavuligerus,” PROTEIN AND PEPTIDE LETTERS, pp. 205–220, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/90388.