Quantitative expression analysis of the genes potentially involved in clavulanic acid overproduction in streptomyces clavuligerus

Aktaş, Caner
Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics, including cephamycin C (CC) and the potent β-lactamase inhibitor clavulanic acid. (CA). We have already undertaken an extensive comparative proteomic analysis by using both 2-DE-MALDI-MS and GeLC-MS approaches between an industrial CA overproducer, namely DEPA and the reference strain NRRL3585. In this context, we documented several differentially expressed (over- and under-represented) proteins accounting for high CA titers in the former strain to include both common and unique ones. In this work, a total of 26 proteins were selected among the proteins that belonged to different functional categories such as secondary metabolite production, regulators of secondary metabolism, primary metabolism, stress response and several unknowns, and subjected to gene expression analysis. Thus, the aim of the present study is to reveal quantitative levels of the transcripts representing the proteins selected among over- and under-represented ones of these categories.


Comparative Transcriptome Analyses of a Standard Strain and Clavulanic Acid Overproducing Industrial Strain of S. clavuligerus
Çelik, Gözde; Özcengiz, Gülay; Son, Çağdaş Devrim; Department of Biotechnology (2021-9)
Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics, including cephamycin C (CC) and potent β-lactamase inhibitor clavulanic acid (CA). In this study, comparative transcriptome analysis between an industrial stain, namely DEPA (CA overproducer strain which was developed by several rounds of classical random mutagenesis), and a reference strain NRLL3585 was performed by using the genome-wide RNA-seq technique. Our aim was to understand and improve the knowledge about the...
Proteomic analysis of two cephamycin c overproducer and an industrial clavulanic acid overproducer strains of streptomyces clavuligerus in comparison with the standard strain NRRL 3585
Ünsaldı, Eser; Özcengiz, Gülay; Department of Biology (2016)
In this study, two cephamycin C (CC) overproducers, Streptomyces clavuligerus AK39 and TB3585 strains, and a clavulanic acid (CA) overproducer S. clavuligerus DEPA were subjected to proteomic analysis to elucidate their differential protein expression profiles when compared to that of the standard strain S. clavuligerus NRRL 3585. Two proteomics approaches were employed: 2DE technique coupled with MALDI-TOF/MS and LC-MS/MS. By using two techniques, a total of 40 proteins were identified as upregulated while...
An integrative-omics analysis of an industrial clavulanic acid-overproducing Streptomyces clavuligerus
KURT KIZILDOĞAN, ASLIHAN; Celik, Gozde; unsaldi, Eser; ÖZCAN, SERVET; AYAZ GÜNER, ŞERİFE; Özcengiz, Gülay (2022-09-01)
Clavulanic acid (CA) is a clinically important secondary metabolite used to treat infectious diseases. We aimed to decipher complex regulatory mechanisms acting in CA biosynthesis by analyzing transcriptome- and proteome-wide alterations in an industrial CA overproducer Streptomyces clavuligerus strain, namely DEPA and its wild-type counterpart NRRL3585. A total of 924 differentially expressed genes (DEGs) and 271 differentially produced proteins (DPPs) were obtained by RNA-seq and nanoLC-MS/MS analyses, re...
Increasing clavulanic acid production both in wild type and industrial streptomyces clavuligerus strains by amplification of positive regulator claR gene
Mutlu, Alper; Özcengiz, Gülay; Department of Biology (2012)
Streptomyces clavuligerus is a Gram-positive, filamentous bacterium which produces several important secondary metabolites, including isopenicillin N, cephamycin C and the β-lactamase inhibitor clavulanic acid. Among these compounds, clavulanic acid is being used in combination with commonly used β-lactam antibiotics in order to fight against bacterial infections that are resistant to such antibiotics. Among these combinations, Augmentin, composed of amoxicillin and clavulanic acid, is the most widely presc...
Reinvestigation of the synthetic and mechanistic aspects of Mn(III) acetate mediated oxidation of enones
Demir, Ayhan Sıtkı; Reis, O; Igdir, AC (Elsevier BV, 2004-04-05)
Mn(OAc)(3) mediated alpha'-acetoxylation of alpha,beta-unsaturated enones is reinvestigated from a synthetic and mechanistic point of view and an improved procedure based on the use of acetic acid as a co-solvent is presented. Excellent results were obtained for a variety of structurally diverse and synthetically important enones under the optimized conditions.
Citation Formats
C. Aktaş, “Quantitative expression analysis of the genes potentially involved in clavulanic acid overproduction in streptomyces clavuligerus,” M.S. - Master of Science, Middle East Technical University, 2018.