Quantitative expression analysis of the genes potentially involved in clavulanic acid overproduction in streptomyces clavuligerus

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2018

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Aktaş, Caner

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Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics, including cephamycin C (CC) and the potent β-lactamase inhibitor clavulanic acid. (CA). We have already undertaken an extensive comparative proteomic analysis by using both 2-DE-MALDI-MS and GeLC-MS approaches between an industrial CA overproducer, namely DEPA and the reference strain NRRL3585. In this context, we documented several differentially expressed (over- and under-represented) proteins accounting for high CA titers in the former strain to include both common and unique ones. In this work, a total of 26 proteins were selected among the proteins that belonged to different functional categories such as secondary metabolite production, regulators of secondary metabolism, primary metabolism, stress response and several unknowns, and subjected to gene expression analysis. Thus, the aim of the present study is to reveal quantitative levels of the transcripts representing the proteins selected among over- and under-represented ones of these categories.

Subject Keywords

Streptomyces., Clavulanic acid., Gene expression.

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http://etd.lib.metu.edu.tr/upload/12622763/index.pdf
https://hdl.handle.net/11511/27666

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

C. Aktaş, “Quantitative expression analysis of the genes potentially involved in clavulanic acid overproduction in streptomyces clavuligerus,” M.S. - Master of Science, Middle East Technical University, 2018.