Combined drought and heat stresses trigger different sets of miRNAs in contrasting potato cultivars

Date

2021-07-01

Author

Gokce, Zahide Neslihan Ozturk
Aksoy, Emre
Bakhsh, Allah
Demirel, Ufuk
Caliskan, Sevgi
Caliskan, Mehmet Emin

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MicroRNAs are small, non-coding RNAs that are responsible for regulation of gene expression during plant growth and development. Although there are many studies on miRNAs in other plants, little work has been done to understand the role of miRNAs in abiotic stress tolerance in potatoes. This study investigates changes in miRNA profiles of two different potato cultivars (tolerant, Unica and susceptible, Russet Burbank) in response to heat, drought and their combination. Transcriptomic studies revealed that miRNA profiles depend on the susceptibility and tolerance of the cultivar and also the stress conditions. Large number of miRNAs were expressed in Unica, whereas Russet Burbank indicated lesser number of changes in miRNA expression. Physiological and transcriptional results clearly supported that Unica cultivar is tolerant to combined drought and heat stress compared to Russet Burbank. Moreover, psRNATarget analysis predicted that major miRNAs identified were targeting genes playing important roles in response to drought and heat stress and their important roles in genetic and post-transcriptional regulation, root development, auxin responses and embryogenesis were also observed. This study focused on eight miRNAs (Novel_8, Novel_9, Novel_105, miR156d-3p, miR160a-5p, miR162a-3p, miR172b-3p and miR398a-5p) and their putative targets where results indicate that they may play a vital role at different post-transcriptional levels against drought and heat stresses. We suggest that miRNA overexpression in plants can lead to increased tolerance against abiotic stresses; furthermore, there should be more emphasis on the studies to investigate the role of miRNAs in combined abiotic stress in plants.

Subject Keywords

MicroRNA, Water defcit, High-temperature stress, Combined stress, Gene regulation, sRNA sequencing

URI

https://hdl.handle.net/11511/92967

Journal

FUNCTIONAL & INTEGRATIVE GENOMICS

DOI

https://doi.org/10.1007/s10142-021-00793-w

Collections

Department of Biology, Article

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Citation Formats

Z. N. O. Gokce, E. Aksoy, A. Bakhsh, U. Demirel, S. Caliskan, and M. E. Caliskan, “Combined drought and heat stresses trigger different sets of miRNAs in contrasting potato cultivars,” FUNCTIONAL & INTEGRATIVE GENOMICS, vol. 21, no. 3-4, pp. 489–502, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/92967.