A protective association between catalase and isocitrate lyase in peroxisomes

Date

2005-03-01

Author

Yanık, Tülin

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Glyoxysomes are specialized peroxisomes in germinating seeds, which catalyze many reactions that convert fatty acids into carbohydrates thus generating H2O2. They are characterized by the presence of catalase (CAT, E.C. 1.11.1.6) in their matrix which protects cells from oxidative stress. Here, we investigated the possibility that a protein can be protected from oxidative damage by its association with CAT. We purified peroxisomal CAT from germinating castor beans by ion exchange, gel filtration, and hydroxylapatite chromatography. Gel filtration of the matrix proteins, cross-linking, and co-immunoprecipitation studies indicate that CAT associates with a glyoxysomal matrix protein, isocitrate lyase (ICL, E.C. 4.1.3.1). In addition, we found that H2O2 inactivates ICL and degrades its product, glyoxylate, when CAT is inactive. ICL and its product appear to be sensitive to oxidative damage; thus, association of CAT with ICL would afford protection from H2O2. (C) 2004 Elsevier Inc. All rights reserved.

URI

https://hdl.handle.net/11511/94086

Journal

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

DOI

https://doi.org/10.1016/j.abb.2004.12.017

Collections

Department of Biology, Article

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Citation Formats

T. Yanık, “A protective association between catalase and isocitrate lyase in peroxisomes,” ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 435, no. 2, pp. 243–252, 2005, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/94086.