A prelude to the proximity interaction mapping of CXXC5

Date

2021-09-01

Author

Ayaz, Gamze
Turan, Gizem
Olgun, Çağla Ece
Kars, Gizem
Karakaya, Burcu
Yavuz, Kerim
Demiralay, Oyku Deniz
Can, Tolga
Muyan, Mesut
Yasar, Pelin

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CXXC5 is a member of the zinc-finger CXXC family proteins that interact with unmodified CpG dinucleotides through a conserved ZF-CXXC domain. CXXC5 is involved in the modulation of gene expressions that lead to alterations in diverse cellular events. However, the underlying mechanism of CXXC5-modulated gene expressions remains unclear. Proteins perform their functions in a network of proteins whose identities and amounts change spatiotemporally in response to various stimuli in a lineage-specific manner. Since CXXC5 lacks an intrinsic transcription regulatory function or enzymatic activity but is a DNA binder, CXXC5 by interacting with proteins could act as a scaffold to establish a chromatin state restrictive or permissive for transcription. To initially address this, we utilized the proximity-dependent biotinylation approach. Proximity interaction partners of CXXC5 include DNA and chromatin modifiers, transcription factors/co-regulators, and RNA processors. Of these, CXXC5 through its CXXC domain interacted with EMD, MAZ, and MeCP2. Furthermore, an interplay between CXXC5 and MeCP2 was critical for a subset of CXXC5 target gene expressions. It appears that CXXC5 may act as a nucleation factor in modulating gene expressions. Providing a prelude for CXXC5 actions, our results could also contribute to a better understanding of CXXC5-mediated cellular processes in physiology and pathophysiology.

URI

https://hdl.handle.net/11511/94187

Journal

SCIENTIFIC REPORTS

DOI

https://doi.org/10.1038/s41598-021-97060-6

Collections

Department of Biology, Article

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Citation Formats

G. Ayaz et al., “A prelude to the proximity interaction mapping of CXXC5,” SCIENTIFIC REPORTS, vol. 11, no. 1, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/94187.