Photofermentative Hydrogen Production by Agar Immobilized Co-Cultures of Purple Non-Sulfur Bacteria

Purple non-sulfur (PNS) bacteria have the ability to produce biohydrogen via photofermentation. This study focuseson hydrogen production using co-cultures of immobilized PNS bacteria, investigates the possible synergistic effectof their co-cultures, and the advantages of immobilization. Growth, hydrogen production and substrate utilization ofsingle-cultures and, double and triple co-cultures of R. capsulatus hup- (YO3), Rp. palustris (DSM 127) and R.sphaeroides O.U.001 (DSM 5864) were compared using an artificial hydrogen production medium in 150 mL indoorsand outdoors photobioreactors on 40/2 Acetate/Glutamate medium under continuous illumination and anaerobicconditions. Hydrogen yields were found to be within 1.71-3.21 mol H2/mol acetate and hydrogen productivities werefound to be within 0.0447-0.406 mol H2/(L.h). Additional studies with different temperature, illumination, andsubstrate concentration parameters need to be carried out for deeper understanding of the mechanism of hydrogenproduction in immobilized co-cultures in order to further improve and standardize hydrogen yields and productivities.
5th International Hydrogen Technologies Congress


Photobiological hydrogen production from sugar beet molasses
Sağır, Emrah; Yücel, Ayşe Meral; Özgür, Ebru; Department of Biochemistry (2012)
The main aim of this study was to investigate biological hydrogen production from sucrose and molasses by purple non-sulphur bacteria (PNS). The hydrogen production capacities of four different PNS bacteria (Rhodobacter capsulatus (DSM 1710), Rhodobacter capsulatus YO3 (Hup-), Rhodopseudomonas palustris (DSM 127) and Rhodobacter sphaeroides O.U.001 (DSM 5864)) were tested on sucrose and molasses. The photobiological hydrogen production were performed in 50 ml and 150 ml small scale photobioreactors, in batc...
Enantioselective Michael addition of malononitrile to chalcone with bifunctional squaramide organocatalysts
Sargın, Nurdan; Tanyeli, Cihangir; Department of Chemistry (2015)
4H-pyran derivatives show diverse biological activity such as antimicrobial, antibacterial, antiviral and antifungal. 4H-pyran derivatives can be synthesized easily from 2-(3-oxo-1,3-diphenylpropyl)malononitrile which is the Michael addition product of malononitrile to trans-chalcone. Synthesizing chiral biologically active compounds with metal free approach (organocatalysis) is a significant topic. In this thesis, chiral 2-(3-oxo-1,3-diphenylpropyl)malononitrile was synthesized with quinine or 2-aminoDMAP ...
Antifungal spectrum determination of the K5 type yeast killer protein on fungi causing spoilage in citrus fruits
Kepekçi, Remziye Aysun; İzgü, Kadri Fatih; Department of Biology (2006)
Some yeast strains under certain conditions secrete polypeptide toxins which are inhibitory to sensitive fungal cells into the medium. These yeast strains are termed as killer yeasts and their toxins are designated as killer proteins or killer toxins. Killer proteins are classified into 11 typical types (K1-K11). These toxins have different killing mechanisms on sensitive cells. Some of them hydrolyze major cell wall component, beta-1,3- glucans. As mammalian cells lack cell walls research and development o...
Chemosensing in deep red: A squaraine-based fluorescent chemosensor for pH.
Isgor, YG; Akkaya, EU (Elsevier BV, 1997-10-20)
We have synthesized and spectrally characterized a squaraine-based fluorescent chemosensor for pH. This chemosensor unlike many others, works in 100 % aqueous solutions, signalling the pH change from 10 to 7 by a 11-fold increase in the emission intensity at 651 nm, when excited at the isosbestic point (614 nm). The average pK(a) for the ionizable groups is found to be 8.8. The chemosensor with its molar extinction coefficient > 200,000 and the quantum yield of 0.2, yield an impressive intrinsic brightness ...
Nanofiltration and Reverse Osmosis for Reuse of Indigo Dye Rinsing Waters
Uzal, Nigmet; Yılmaz, Levent; Yetiş, Ülkü (Informa UK Limited, 2010-01-01)
A membrane based treatment strategy was developed for the possible recycling of rinsing wastewater from indigo dyeing to the process itself. Performances of three different nanofiltration (NF) (NF 270 and NF 90, Dow Film Tech, USA and NF 99, Alfa Laval, Denmark) and two different reverse osmosis (RO) (HR 98 PP and CA 995 PE, Alfa Laval, Denmark) membranes were investigated with wastewater collected from the first post-rinsing tank of indigo dyeing process of a denim manufacturing plant. Dead-end microfiltra...
Citation Formats
H. Koku and A. M. Yücel, “Photofermentative Hydrogen Production by Agar Immobilized Co-Cultures of Purple Non-Sulfur Bacteria,” presented at the 5th International Hydrogen Technologies Congress, Niğde, Türkiye, 2021, Accessed: 00, 2021. [Online]. Available: