Succinic acid production from cheese whey via fermentation by using alginate immobilized Actinobacillus succinogenes

2021-12-01
Uysal, Utku
Hamamcı, Haluk
© 2021 Elsevier LtdThe production of succinic acid from cheese whey was studied with immobilized and free cells of Actinobacillus succinogenes. Alginate was used as immobilizing agent, the durability of alginate beads was assessed and improved. Batch fermentations with free cells were conducted using cheese whey, lactose and glucose, highest succinic acid production was achieved with cheese whey (24.9 g L−1) with 35 g L−1 starting lactose concentration. For immobilized batch fermentations, 74.9% yield and 1.09 g L−1 h−1 productivity achieved with cheese whey. Repeated-batch fermentations conducted with whey using the same beads in 5 repeated cycles (144 h) and 0.89 g L−1 h−1 of productivity was achieved. For continuous fermentations with whey and immobilized cells, maximum 0.84 g/g yield was achieved. In this work, it is suggested that immobilizing A. succinogenes with alginate-whey mixture is an effective technique and cheese whey is a valuable resource for the production of succinic acid.
Bioresource Technology Reports

Suggestions

IMMOBILIZATION OF ACTINOBACILLUS SUCCINOGENES BY USING ALGINATE FOR SUCCINIC ACID PRODUCTION FROM DAIRY WASTES
Uysal, Utku; Çekmecelioğlu, Deniz; Hamamcı, Haluk; Department of Food Engineering (2022-2-02)
Succinic acid is a highly versatile building block that is used in a wide range of industrial applications. The biological production of succinic acid has emerged in the last years as an efficient alternative to the chemical production based on fossil fuels. However, in order to fully replace the competing petro-based chemical process from which it has been produced so far, some challenges remain to be surpassed. In particular, one main obstacle would be to reduce its production costs, mostly associated to ...
Selenium speciation in chicken breast samples from inorganic and organic selenium fed chickens using high performance liquid chromatography-inductively coupled plasma-mass spectrometry
BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2018-08-01)
Speciation of selenium in chicken breast samples was performed by anion and cation exchange HPLC systems combined with ICP-MS. Samples were categorized into three groups consisting of Control Group, Inorganic Se Fed Group and Organic Se Fed Group. After the optimization of speciation parameters, selenomethionine, selenocystine, Se(IV) and Se(VI) were determined in the samples using the HPLC-ICP-MS system. Instrumental limit of detection values for selenium(IV), selenium(VI), Se in selenomethionine and Se in...
Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads
ALTINOK, HAYDAR; AKSOY, SERPİL; TÜMTÜRK, HAYRETTİN; Hasırcı, Nesrin (2008-06-01)
A carrier for invertase enzyme was synthesized from styrene (S) and 2- hydroxyethyl methacrylate (HEMA) in the form of microbeads. These poly (styrene-2-hydroxyethyl methacrylate), P(S-HEMA) microbeads were activated by epichlorohydrin (ECH) treatment for covalent immobilization. The free and immobilized invertase were assayed in the hydrolysis of sucrose to glucose, and the obtained results were compared. The optimum pH was 4.5 for free and 5.5 for immobilized invertase. The optimum temperature of invertas...
Nanostructured chitosan/monolaurin film: Preparation, characterization and antimicrobial activity against Listeria monocytogenes on ultrafiltered white cheese
Lotfi, Mohamad; Tajik, Hossein; Moradi, Mehran; Forough, Mehrdad; Divsalar, Elahe; Kuswandi, Bambang (2018-06-01)
Antimicrobial cellulose-chitosan (CC) films containing monolaurin (ML) (0.5 and 1%) were developed using the sol-gel method and characterized in terms of microscopic, structural, swelling, water solubility, and antimicrobial properties against Listeria monocytogenes in vitro and on ultrafiltered cheese during storage at 4 for 14 days. The results of antimicrobial activity revealed that the addition of ML significantly (p < 0.05) increased the diameter of the inhibition zone. Moreover, CC film did not show i...
Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry
BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2015-06-13)
Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in sele...
Citation Formats
U. Uysal and H. Hamamcı, “Succinic acid production from cheese whey via fermentation by using alginate immobilized Actinobacillus succinogenes,” Bioresource Technology Reports, vol. 16, pp. 0–0, 2021, Accessed: 00, 2022. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85121124297&origin=inward.