Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry

Date

2015-06-13

Author

BAKIRDERE, Sezgin
Volkan, Mürvet
Ataman, Osman Yavuz

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Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in selenocystine were 0.85, 0.68, 0.84, and 0.99 nanogram per milliliter, respectively. Six brands were analyzed to identify and quantify the selenium species present, and the results found were compared with the values given on the labels. The selenium species matched the labeled species for four brands, whereas two brands were found to contain inorganic Se(VI) in contrast with the labeled claim of selenomethionine.

Subject Keywords

High-performance liquid chromatography, Inductively coupled plasma-mass spectrometry, Speciation, Selenium, Vitamin, HPLC-ICP-MS

URI

https://hdl.handle.net/11511/31981

Journal

ANALYTICAL LETTERS

DOI

https://doi.org/10.1080/00032719.2014.986679

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

S. BAKIRDERE, M. Volkan, and O. Y. Ataman, “Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry,” ANALYTICAL LETTERS, pp. 1511–1523, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31981.