Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry

Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in selenocystine were 0.85, 0.68, 0.84, and 0.99 nanogram per milliliter, respectively. Six brands were analyzed to identify and quantify the selenium species present, and the results found were compared with the values given on the labels. The selenium species matched the labeled species for four brands, whereas two brands were found to contain inorganic Se(VI) in contrast with the labeled claim of selenomethionine.


Speciation of Arsenic in Fish by High-Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry
Ozcan, Sefika; BAKIRDERE, Sezgin; Ataman, Osman Yavuz (2016-01-01)
Arsenic speciation in fish was performed using isocratic elution with cation exchange column high-performance liquid chromatography and inductively coupled plasma-mass spectrometry detection. The separation of arsenobetaine and dimethylarsinic acid was performed in 12 min. DOLT-4 dogfish liver and NIST RM-50 albacore tuna certified reference materials were used to evaluate the accuracy for the determination of total arsenic in fish. In addition, reference materials were analyzed for arsenobetaine and dimeth...
Selenium speciation in chicken breast samples from inorganic and organic selenium fed chickens using high performance liquid chromatography-inductively coupled plasma-mass spectrometry
BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2018-08-01)
Speciation of selenium in chicken breast samples was performed by anion and cation exchange HPLC systems combined with ICP-MS. Samples were categorized into three groups consisting of Control Group, Inorganic Se Fed Group and Organic Se Fed Group. After the optimization of speciation parameters, selenomethionine, selenocystine, Se(IV) and Se(VI) were determined in the samples using the HPLC-ICP-MS system. Instrumental limit of detection values for selenium(IV), selenium(VI), Se in selenomethionine and Se in...
Enantioselective chemoenzymatic synthesis of oseltamivir (tamiflu)(r) intermediates
Eşiyok, Hacı; Demir, Ayhan Sıtkı; Department of Biotechnology (2008)
The objective of this presented study was to synthesize optically active compounds considered to be key intermediates in the synthesis of Oseltamivir (Tamiflu) by performing chemical and biotechnological methods. Thereof, the carboethoxy cyclohexenone skeleton first was synthesized utilizing easily available substances. The synthesis of alpha-hydroxy ketones in enantiomerically pure form offers a great importance in the synthesis of biologically active compounds. Toward this fact, the enantioselective synth...
Biological hydrogen production from olive mill wastewater and its applications to bioremediation
Eroğlu, Ela; Eroğlu, İnci; Department of Chemical Engineering (2006)
Hydrogen production by photosynthetic bacteria occurs under illumination in the presence of anaerobic atmosphere from the breakdown of organic substrates, which is known as photofermentation. In this study, single-stage and two-stage process development were investigated for photofermentative hydrogen production from olive mill wastewater by Rhodobacter sphaeroides O.U.001 within indoor and outdoor photobioreactors. It was proven that diluted olive mill wastewater (OMW) could be utilized for photobiological...
Spectroscopic investigation of oxidation of p-toluene sulfonic acid doped polypyrrole
Uyar, Tamer; Toppare, Levent Kamil; Hacaloğlu, Jale (2001-09-04)
Oxidative degradation of electrochemically synthesized p-toluene sulfonic acid doped polypyrrole, PTS-PPY was studied by the application of evolved gas analysis using IR spectroscopy and direct insertion probe pyrolysis mass spectrometry techniques. It has been observed that PPY films prepared in aqueous media contained high concentration of oxygenated species even if the synthesis potential was kept low. The extent of overoxidation associated to ketone formation increased by increasing the applied potentia...
Citation Formats
S. BAKIRDERE, M. Volkan, and O. Y. Ataman, “Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry,” ANALYTICAL LETTERS, pp. 1511–1523, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31981.