Selenium speciation in chicken breast samples from inorganic and organic selenium fed chickens using high performance liquid chromatography-inductively coupled plasma-mass spectrometry

Speciation of selenium in chicken breast samples was performed by anion and cation exchange HPLC systems combined with ICP-MS. Samples were categorized into three groups consisting of Control Group, Inorganic Se Fed Group and Organic Se Fed Group. After the optimization of speciation parameters, selenomethionine, selenocystine, Se(IV) and Se(VI) were determined in the samples using the HPLC-ICP-MS system. Instrumental limit of detection values for selenium(IV), selenium(VI), Se in selenomethionine and Se in selenocystine were found to be 0.75, 0.80, 0.55 and 0.46 ng mL(-1), respectively. Protease XIV enzyme in 30.0 mM Tris HCl buffered to pH 7.2 was used in the extraction of selenium species from the sample matrix. Total selenium concentrations in the Control, Inorganic Se Fed and Organic Se Fed groups were found to be 675 +/- 85, 1084 +/- 198 and 887 +/- 139 ng g(-1) based on dry mass, respectively. The concentration of selenomethionine in the Organic Se Fed Group was found to be higher than those in the Control and Inorganic Se Fed Groups. Se(IV) was determined only in the Inorganic Se Fed Group.


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BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2015-06-13)
Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in sele...
OZILGEN, M (Wiley, 1993-02-01)
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Arsenic speciation in fish was performed using isocratic elution with cation exchange column high-performance liquid chromatography and inductively coupled plasma-mass spectrometry detection. The separation of arsenobetaine and dimethylarsinic acid was performed in 12 min. DOLT-4 dogfish liver and NIST RM-50 albacore tuna certified reference materials were used to evaluate the accuracy for the determination of total arsenic in fish. In addition, reference materials were analyzed for arsenobetaine and dimeth...
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The role of $f_{0}(980)$ and $a_{0}(980)$ scalar meson intermediate states in the mechanism of radiative $\phi (1020)$ meson decay into two charged $K (494)$ mesons and a photon $\phi\rightarrow K^{+} + K^{-} + \gamma$ is investigated. For the contribution of scalar meson intermediate state two models are considered. In the kaon-loop model, the scalar meson intermediate state couples the final state to the initial $\phi$ meson through a charged kaon-loop. The second model, called no-structure model, consist...
Citation Formats
S. BAKIRDERE, M. Volkan, and O. Y. Ataman, “Selenium speciation in chicken breast samples from inorganic and organic selenium fed chickens using high performance liquid chromatography-inductively coupled plasma-mass spectrometry,” JOURNAL OF FOOD COMPOSITION AND ANALYSIS, pp. 28–35, 2018, Accessed: 00, 2020. [Online]. Available: