Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Evaluation of Performance of Arsenic Bioreporter Immobilized Electrospun Membranes for Arsenic Detection in Water
Download
10511952_Nehir Arık Kınalı.pdf
Date
2022-12-9
Author
Arık Kınalı, Nehir
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
189
views
53
downloads
Cite This
Heavy metal pollution, which is growing and threatening the health of all living things as well as human health, can lead to serious lifelong consequences. It is very important to detect and monitor these heavy metals that cause environmental pollution. In addition to traditional laboratory techniques, the use of bacterial biosensors to detect heavy metals has come into focus. In addition, it is known that the immobilization of these bacterial biosensors is more advantageous in many respects, such as being more stable and protected than the free ones. In this thesis study, arsenic bioreporters whose expression of the reporter gene increased in the presence of arsenic and which we could record as fluorescent signal, were immobilized on 12.5% cellulose acetate (CA) and 10 wt% polycaprolactone (PCL) electrospun fibers. The characterization tests of the developed electrospun fibers were carried out by means of scanning electron microscopy (SEM), fourier-transform infrared (FTIR) spectroscopy and contact angle meter. It was observed that the developed fiber support membranes were beadless and homogeneous fibers (Diameters: 2.15 μm ±0.55 for CA; 0.61 μm ±0.20 for PCL) and both were hydrophobic (143.6°±13.5 for CA, 106°±0.3 for PCL). The parameters of the immobilization studies were changed, and their optimization was tried to be achieved. For this, experiments were carried out in different conditions to determine the relationship between the fluorescent signal emitted in the presence of arsenic and the concentrations and growth phases of the immobilized cells. In addition, according to the viability test performed by AlamarBlue method, the viability of the bioreporters immobilized on CA fiber was 55.4%, while those immobilized-on PCL fiber were 91%. Afterwards, the studies were continued on the PCL fiber and the effects of the growth medium and different immobilization times on the fluorescent signal were examined and the properties of the bioreporter immobilized on PCL system were tried to be determined. Following the optimization studies, in order to determine the properties of the bioreporter immobilized on PCL system, the sensitivity, selectivity, response time to arsenic metal and storage life were determined by changing the storage conditions. The study results show that the bioreporter immobilized on PCL system can be used to detect arsenic heavy metal at environmentally safe concentrations. In addition, it is thought that the study will fill the deficiencies in this field in the literature and shed light on future studies.
Subject Keywords
Arsenic
,
Electrospun Fibers
,
Whole Cell Bioreporter
,
Adsorption
,
Bacteria Immobilization
URI
https://hdl.handle.net/11511/101285
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Development of fluorescent whole-cell bacterial bioreporters for detection of inorganic arsenic and cadmium
Elçin, Evrim; Öktem, Hüseyin Avni; Department of Biotechnology (2019)
Environmental heavy metal contamination in many regions of the world is a serious problem of ecological health. Fast and constant monitoring of their levels is significant for both preventing their accumulation and taking an immediate action for removal. As an alternative approach to standard laboratory techniques, various biosensor systems for detection of heavy metals have been proposed. Bacterial biosensors hold great promise for in-field detection of heavy metals. In this study, two different whole cell...
Determination of the optimum loading strategies for monochloro-, trichloro-, and 2,4-dichlorophenoxyacetic acids to anaerobic cultures
GÜVEN, ENGİN; Bayramoğlu, Tuba Hande; Demirer, Göksel Niyazi (2000-01-01)
With respect to their adverse health and environmental effects, halogenated organic compounds (HOCs) are among the most important priority pollutants. HOCs cannot be easily biodegraded. However. if suitable microbial cultures are acclimated to these compounds properly, and the optimum operating conditions are achieved, HOCs can be removed with biological methods. Recently, great interest has focused on reductive anaerobic dehalogenation for the removal of these compounds. This process yields lower halogenat...
Development of a glutathione-S-transferase-based biosensor for the detection of heavy metals
Saatçi, Ebru; İşcan, Mesude; Department of Biochemistry (2005)
In the recent years, environmental pollution becomes a health threatening issue for human beings. Technological developments introduce industrial wastes and heavy metals, and developments in agriculture introduce pesticides into the world that we live. All these toxic wastes accumulate in drinking water and food consumed by humans. Therefore, detection of toxic wastes in all kinds of environmental samples, and development of new detection techniques become an important issue. In this study, development of a...
Assessment of PCB contamination, the potential for in situ microbial dechlorination and natural attenuation in an urban watershed at the East Coast of the United States
Kaya, Devrim; Sowers, Kevin R.; Demirtepe, Hale; Stiell, Brian; Baker, Joel E.; İmamoğlu, İpek; Kjellerup, Birthe (Elsevier BV, 2019-09-15)
Sediment contamination is a major environmental issue in many urban watersheds and coastal areas due to the potential toxic effects of contaminants on biota and human health. Characterizing and delineating areas of sediment contamination and toxicity are important goals of coastal resource management in terms of ecological and economical perspectives. Core and surficial sediment samples were collected from an industrialized urban watershed at the East Coast of the United Stated and analyzed to evaluate the ...
Molecular adaptations in cadmium and lead resistant environmental species
Kepenek, Eda Şeyma; Gözen, Ayşe Gül; Severcan, Feride; Department of Biology (2017)
Environmental pollution caused by heavy metal exposure has detrimental effects on human health. For the reclamation of polluted areas, bacteria have been used in remediation. For those bioremediation attempts, finding the appropriate bacterial strains is an important issue. In their environment, bacteria may exist close to the source of heavy metals. Sudden exposure to high concentrations of the heavy metal may trigger quick response mechanisms to ensure survival. On the other hand, bacteria may live at a d...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
N. Arık Kınalı, “Evaluation of Performance of Arsenic Bioreporter Immobilized Electrospun Membranes for Arsenic Detection in Water,” Ph.D. - Doctoral Program, Middle East Technical University, 2022.