Enhanced antimicrobial activity of volatile thymol molecules encapsulated in hierarchical titanosilicates

Binay, Melda Isler
Akata Kurç, Burcu
Realizing higher loading and extended-release time of bulkier molecules for enhanced antibacterial activity can be a challenge, which is highly dependent on the pore characteristics of the network used for their encapsulation. This study highlights that the hierarchical microporous Engelhard titanosilicate ETS-10 crystals support a higher amount of thymol encapsulation to allow long-term thymol release and higher antimicrobial activity. In this study, post-synthesis treatment of ETS-10 was performed with H2O2 at two different concentrations to induce hierarchical porosity resulting in a gradual increase in surface area and pore volume of ETS-10. The thymol encapsulated and post-treated ETS-10 crystals with enlarged porosity were shown to exhibit enhanced antimicrobial activity against P. aeruginosa, S. aureus, and C. albicans compared to untreated ones. Furthermore, the thymol release time was prolonged significantly in addition to an altered release profile. Our findings suggest that tailoring pore characteristics by a simple post-treatment applied to microporous networks creating some hierarchical structure can be an alternative approach for enhanced antimicrobial activity and prolonged release of oil extracts that are vulnerable to environmental conditions.


Enhanced recombinant human growth hormone production by synchronous populations of two different strains of pichia pastoris under GAP promoter
Hüccetoğulları, Damla; Çalık, Pınar; İnan, Mehmet; Department of Biotechnology (2016)
Cell cycle synchronization used as a common procedure for understanding various genomic and metabolic phenomena in a cell is also an essential tool to optimize the production of biomolecules. In this work, the effect of cell cycle synchronization on the recombinant human growth hormone (rhGH) production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter was investigated. Starvation as a cost effective and readily applicable synchronization method was performed to investigate va...
Studies towards the preparation of functionalized cellulose microspheres with affinities towards negatively charged biomembranes
Demirel, Kaan; Türkyılmaz, Serhan; Department of Chemistry (2021-7)
Cellulose microspheres (CMs), generally with diameters in the 10-1000 µm range, have been used as stationary phases in chromatography, substrates for ion exchange applications, supports for protein immobilization and solid-phase synthesis, and drug loading and release materials. Furthermore, cellulose and CMs are biocompatible and have been used in many biomedical applications as varied as the dressing of wounds and blood filtration. Targeting of bacterial cells can be achieved using antibodies, antibody fr...
A high throughput approach for analysis of cell nuclear deformability at single cell level
Ermis, Menekse; Akkaynak, Derya; Chen, Pu; Demirci, Utkan; Hasırcı, Vasıf Nejat (2016-11-14)
Various physiological and pathological processes, such as cell differentiation, migration, attachment, and metastasis are highly dependent on nuclear elasticity. Nuclear morphology directly reflects the elasticity of the nucleus. We propose that quantification of changes in nuclear morphology on surfaces with defined topography will enable us to assess nuclear elasticity and deformability. Here, we used soft lithography techniques to produce 3 dimensional (3-D) cell culture substrates decorated with micron ...
Hatiboğlu, Anıl; Külah, Haluk; Önel, Selis; Department of Micro and Nanotechnology (2021-9-13)
Modeling cell membrane permeability in different solutions is a critical requirement in controlling the response of cells during preconcentration processes in biotechnological applications, such as drug delivery, fluorescence imaging, and cryopreservation . Current multi-step methods employed in loading cells with high concentrations of cryoprotectant agents (CPAs) prior to cryopreservation for long term storage affect cell viability as a result of extended exposure times associated with these methods. One ...
Preparation and characterization of hydrogen-bonded layer-by-layer polymer films containing magnetic nanoparticles
Khan, Muhammad Alyaan Ahmed; Erel Göktepe, İrem; Department of Polymer Science and Technology (2014)
Stimuli responsive layer-by-layer (LbL) polymer films are promising materials as platforms for controlled release of functional biological molecules such as drugs, proteins, growth hormones, etc. from surfaces. Recently, there has been a growing interest for preparation of LbL polymer platforms containing superparamagnetic iron oxide nanoparticles for dual functionality, i.e. bioimaging and controlled delivery of biological molecules. Moreover, if superparamagnetic iron oxide nanoparticles are embedded into...
Citation Formats
M. I. Binay, D. KART, and B. Akata Kurç, “Enhanced antimicrobial activity of volatile thymol molecules encapsulated in hierarchical titanosilicates,” JOURNAL OF POROUS MATERIALS, pp. 0–0, 2022, Accessed: 00, 2023. [Online]. Available: https://hdl.handle.net/11511/101504.