Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Studies towards the preparation of functionalized cellulose microspheres with affinities towards negatively charged biomembranes
Download
Demirel_Kaan_Master_Thesis.pdf
Date
2021-7
Author
Demirel, Kaan
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
381
views
65
downloads
Cite This
Cellulose microspheres (CMs), generally with diameters in the 10-1000 µm range, have been used as stationary phases in chromatography, substrates for ion exchange applications, supports for protein immobilization and solid-phase synthesis, and drug loading and release materials. Furthermore, cellulose and CMs are biocompatible and have been used in many biomedical applications as varied as the dressing of wounds and blood filtration. Targeting of bacterial cells can be achieved using antibodies, antibody fragments, peptides, polycationic dendrimers, antibiotics such as vancomycin, and certain metal complexes. Zinc (II) bisdipicolylamine (Zn2BDPA) complexes are capable of selectively binding to negatively charged phosphate amphiphiles displayed by bacterial cells. Blood is normally sterile and the presence of bacteria in blood is a condition known as bacteremia. Bacteremia can be caused by burns, injuries, local infections, surgical procedures, and the use of catheters or needles. If undetected and untreated, the immune response to bacteremia can result in sepsis, septic shock, and multiple organvi failure. Septic shock and multiple organ failure as a result of hospital-acquired bacteremia is a leading cause of death in intensive care units. This study aimed to develop Zn2BDPA-derivatized CMs as selective sorbents capable of binding to negatively charged liposomes and bacterial cells, with an ultimate goal of obtaining functional materials for the treatment of bacteremia through extracorporeal whole blood filtration. To this end, the preparation of CMs through the thermal regeneration of cellulose from water-in-oil emulsions of viscose has been investigated and the effect of various factors on particle size have been determined. Underivatized (native) CMs were then activated to display aldehyde, amino, carboxylic acid, cyano, and epoxy functionalities. Amine and carboxylic acid bearing BDPA ligands were then coupled with some of these activated CMs. Formation of BDPA-CM conjugates was confirmed using several analytical techniques. Zn2BDPA complex functionalized CMs were then shown to bind to negatively charged fluorescent liposomes and green fluorescent protein (GFP) expressing bacterial cells through fluorescence spectroscopic measurements and/or fluorescence microscopy. Further development of these materials is likely to lead to effective sorbents for bacterial cells which would have applications in the diagnosis and treatment of bacteremia and in the isolation and preconcentration of bacterial cells from a variety of liquid samples.
Subject Keywords
Cellulose
,
Cellulose Microspheres
,
Bacteremia
,
Bacterial Targeting
URI
https://hdl.handle.net/11511/92023
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Studies Towards the Preparation of Functional Cellulose Microspheres with an Affinity Towards Bacterial Cells
Demirel, Kaan; Akbulut, Doğan; Yılmaz, Ozan; Türkyılmaz, Serhan (2021-04-08)
Cellulose microspheres (CMs, diameters 10-1000 m) have been used as stationary phases in chromatography, substrates for ion exchange applications, supports for protein immobilization and solid-phase synthesis, and drug loading and release materials.2 Furthermore CMs are biocompatible and have been used in many biomedical applications as varied as blood filtration3,4 and the dressing of wounds. Blood is normally sterile and the presence of bacteria in blood is a condition known as bacteremia. Bacteremia can...
Pesticide and model drug release from carboxymethylceullose microspheres
Darvari, R; Hasırcı, Vasıf Nejat (1996-01-01)
Water soluble derivatives of cellulose are widely used in various biomedical and biotechnological applications. Sodium carboxymethyl cellulose was insolubilized in the form of microspheres using aluminium chloride as the crosslinking agent. It was observed that, depending on the preparation medium pH, the spherical product could either be a microsphere with an ionotropic interior or a microcapsule. Various microspheres with different crosslinker, biopolymer, and drug (2',7'-dichlorofluorescein and aldicarb)...
Integration of a Glutamate Sensitive Genetically Encoded Sensor Protein into Photocrosslinkable Hydrogel Optrodes
Kahyaoğlu, Leyla Nesrin (2016-01-01)
Immobilization into 3D matrices stabilizes proteins in comparison to flat planar surfaces and facilitates the study of the biomolecular interactions as well as integration into optrodes for cell physiology. Photocrosslinkable hydrogels have received significant attention in recent years as they provide not only a highly hydrophilic 3D environment to promote protein stabilization and its interactions with analyte molecules, but enable optically addressable patterning for spatial control of protein localizati...
Investigating the malleability of RNA aptamers
İlgü, Müslüm; Lamm, Monica H.; Nilsen-Hamilton, Marit (Elsevier BV, 2013-09-15)
Aptamers are short, single-stranded nucleic acids with structures that frequently change upon ligand binding and are sensitive to the ionic environment. To achieve facile application of aptamers in controlling cellular activities, a better understanding is needed of aptamer ligand binding parameters, structures, intramolecular mobilities and how these structures adapt to different ionic environments with consequent effects on their ligand binding characteristics. Here we discuss the integration of biochemic...
Study on the properties of crosslinking of poly(ethylene oxide) and hydroxyapatite-poly(ethylene oxide) composite
Banat, R; Tincer, T (2003-10-10)
This study covers the crosslinking of poly-(ethylene oxide) (PEO) and its composite with calcium hydroxyapatite (HA), their mechanical and swelling properties, and morphology. Sheets of the composites of PEO (two different grades with M-v: 5x10(6) and 2x10(5)) and HA and neat PEO were prepared by compression molding. The prepared composite. and. PEO (0.1-mm-thick) sheets were crosslinked with exposure of UV-irradiation in the presence of a photoinitiator, acetophenone (AP). This simple method for crosslinki...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
K. Demirel, “Studies towards the preparation of functionalized cellulose microspheres with affinities towards negatively charged biomembranes,” M.S. - Master of Science, Middle East Technical University, 2021.