Injectable methacrylated gelatin/thiolated pectin hydrogels carrying melatonin/tideglusib-loaded core/shell PMMA/silk fibroin electrospun fibers for vital pulp regeneration

Date

2023-02-01

Author

Atila, Deniz
Keskin, Dilek
Lee, Yuan-Ling
Lin, Feng-Huei
Hasirci, Vasif
Tezcaner, Ayşen

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Use of injectable hydrogels attract attention in the regeneration of dental pulp due to their ability to fill non-uniform voids such as pulp cavities. Here, gelatin methacrylate/thiolated pectin hydrogels (GelMA/PecTH) carrying electrospun core/shell fibers of melatonin (Mel)-polymethylmethacrylate (PMMA)/Tideglusib (Td)-silk fibroin (SF) were designed as an injectable hydrogel for vital pulp regeneration, through prolonged release of Td and Mel to induce proliferation and odontoblastic differentiation of dental pulp stem cells (DPSC). H NMR and FTIR confirmed methacrylation of Gel and thiolation of Pec. Addition of PMMA/SF increased degradation and water retention capacities of GelMA/PecTH. Rheological analyses and syringe tests proved the injectability of the hydrogel systems. Release studies indicated that Td and Mel were released from the fibers inside the hydrogels sequentially due to their specific locations. This release pattern from the hydrogels resulted in DPSC proliferation and odontogenic differentiation in vitro. Gene expression studies showed that the upregulation of DMP1, DSPP, and Axin-2 genes was promoted by GelMA/PecTH carrying PMMA/SF loaded with Mel (50 mu g/mL) and Td (200 nM), respectively. Our results suggest that this hydrogel system holds promise for use in the regeneration of pulp tissue.

Subject Keywords

Injectable hydrogel, Methacrylated gelatin, Thiolated pectin, Core, shell electrospun fiber, Melatonin and tideglusib dual release, DENTAL-PULP, DIFFERENTIATION, MELATONIN, GELATIN, PROLIFERATION, TIDEGLUSIB, INHIBITOR, SCAFFOLDS, TISSUE, CELLS

URI

https://hdl.handle.net/11511/101615

Journal

COLLOIDS AND SURFACES B-BIOINTERFACES

DOI

https://doi.org/10.1016/j.colsurfb.2022.113078

Collections

Department of Engineering Sciences, Article

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Citation Formats

D. Atila, D. Keskin, Y.-L. Lee, F.-H. Lin, V. Hasirci, and A. Tezcaner, “Injectable methacrylated gelatin/thiolated pectin hydrogels carrying melatonin/tideglusib-loaded core/shell PMMA/silk fibroin electrospun fibers for vital pulp regeneration,” COLLOIDS AND SURFACES B-BIOINTERFACES, vol. 222, pp. 0–0, 2023, Accessed: 00, 2023. [Online]. Available: https://hdl.handle.net/11511/101615.