In Vivo Performance of Poly(epsilon-caprolactone) Constructs Loaded with Gentamicin Releasing Composite Microspheres for Use in Bone Regeneration

Sezer, Umran Aydemir
Huri, Pinar Yilgor
Terzioglu, Hakan
Konukseven, Erhan İlhan
Hasırcı, Vasıf Nejat
Hasırcı, Nesrin
For materials used in the production of dental and orthopedic implants or scaffolds for bone tissue regeneration, the properties such as capacity to enhance cell attachment and proliferation, and antimicrobial activity to prevent biofilm formation are very important to improve the clinical utility of the material. In this study, poly(epsilon-caprolactone) (PCL) sponges with antimicrobial activity were prepared by incorporating gentamicin loaded beta-tricalcium phosphate (beta-TCP)-Gelatin microspheres, and in vivo performances were studied. These composite systems are expected to enhance bone regeneration due to beta-TCP and prevent a possible infection by releasing gentannicin in the host location. The effects of gentamicin and beta-TCP/Gelatin microspheres in the sponge structure were studied in vivo by applying them on iliac crest defects of rabbits. Histological analyses after 8 weeks of implantation showed that the composite constructs performed significantly better in bone healing than those with antibiotic-free microspheres. Also, the PCL constructs carrying beta-TCP/Gelatin microspheres led to better bone formation than the pristine PCL scaffolds. Push-out tests demonstrated better integration of the constructs with the tissue indicating high level of material-tissue integration. This study indicates the importance of the presence of antibiotics and beta-TOP/Gelatin in the scaffolds to achieve better and faster healing in bone defects than pristine scaffolds.


Preparation and characterization of poly(epsilon-caprolactone) scaffolds modified with cell-loaded fibrin gel
Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Nesrin (2019-03-15)
Poly(epsilon-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and modified by addition of fibrinogen in order to form a fibrin network between the PCL fibers. By this way, scaffolds would have micro and nanofibers in their structures. Drying of the wet s...
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Korkusuz, F; Korkusuz, P; Eksioglu, F; Gursel, I; Hasırcı, Vasıf Nejat (2001-05-01)
In this study, the major goal was to evaluate in vitro and in vivo findings by macroscopy, radiology, and histology to determine the effectiveness of therapy of experimental implant-related osteomyelitis with antibiotic carrier rods constructed of microbial polyesters. The polymers used were poly(3-hydroxybutyrate-co-4-hydroxyvalerate) [P(3HB-co-4-HB)] and poly(3-hydroxybutyrate-co-3-hydroxy-valerate) [P(3-HB-co-3-HV)]. Both the Sulperazone(R) and the Duocid(R)-P(3-HB-co-4-HB) rods with a drug to polymer ra...
PCL-TCP wet spun scaffolds carrying antibiotic-loaded microspheres for bone tissue engineering
Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2018-01-01)
Scaffolds produced for tissue engineering applications are proven to be promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, porous and fibrous poly(epsilon-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of tricalcium phosphate (TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial ...
The role of multiple toll-like receptor signalling cascades on interactions between biomedical polymers and dendritic cells
Shokouhi, Behnaz; Coban, Cevayir; Hasırcı, Vasıf Nejat; Aydin, Erkin; Dhanasingh, Anandhan; Shi, Nian; Koyama, Shohei; Akira, Shizuo; Zenke, Martin; Sechi, Antonio S. (2010-08-01)
Biomaterials are used in several health-related applications ranging from tissue regeneration to antigen-delivery systems. Yet, biomaterials often cause inflammatory reactions suggesting that they profoundly alter the homeostasis of host immune cells such as dendritic cells (DCs). Thus, there is a major need to understand how biomaterials affect the function of these cells. In this study, we have analysed the influence of chemically and physically diverse biomaterials on DCs using several murine knockouts. ...
Advanced cell therapies with and without scaffolds
Demirbag, Birsen; HURİ, PINAR; KÖSE, GAMZE; Buyuksungur, Arda; Hasırcı, Vasıf Nejat (2011-12-01)
Tissue engineering and regenerative medicine aim to produce tissue substitutes to restore lost functions of tissues and organs. This includes cell therapies, induction of tissue/organ regeneration by biologically active molecules, or transplantation of in vitro grown tissues. This review article discusses advanced cell therapies that make use of scaffolds and scaffold-free approaches. The first part of this article covers the basic characteristics of scaffolds, including characteristics of scaffold material...
Citation Formats
U. A. Sezer et al., “In Vivo Performance of Poly(epsilon-caprolactone) Constructs Loaded with Gentamicin Releasing Composite Microspheres for Use in Bone Regeneration,” JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, pp. 786–795, 2014, Accessed: 00, 2020. [Online]. Available: