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In vitro evaluation of injectable Tideglusib-loaded hyaluronic acid hydrogels incorporated with Rg1-loaded chitosan microspheres for vital pulp regeneration
Date
2022-02-01
Author
Atila, Deniz
Chen, Ching-Yun
Lin, Chun-Pin
Lee, Yuan-Ling
HASIRCI, Vasıf Nejat
Tezcaner, Ayşen
Lin, Feng-Huei
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Injectable systems receive attention in endodontics due to the complicated and irregular anatomical structure of root canals. Here, injectable Tideglusib (Td)-loaded hyaluronic acid hydrogels (HAH) incorporated with Rg1loaded chitosan microspheres (CSM) were developed for vital pulp regeneration, providing release of Td and Rg1 to trigger odontoblastic differentiation of human dental pulp stem cells (DPSC) by Td and vascularization of pulp by Rg1. The optimal concentrations were determined as 90 nM and 50 mu g/mL for Td and Rg1, and loaded in HA and CSM in HAH, respectively. Odontogenic (COL1A1, ALP, OCN, Axin-2, DSPP, and DMP1) and angiogenic (VEGFA, VEGFR2, and eNOS) differentiation of DPSC cultured in the presence of hydrogels was shown at gene expression level. Our results suggest that our injectable hydrogel formulation has potential to improve strategies for vital pulp regeneration. In vivo evaluations are needed to test the feasibility and potential of these hydrogels for vital pulp regeneration.
Subject Keywords
Injectable hydrogels
,
Hyaluronic acid
,
Chitosan microspheres
,
Tideglusib
,
Rg1
,
Vital pulp regeneration
,
NITRIC-OXIDE SYNTHASE
,
GENE-EXPRESSION
,
GINSENOSIDE RG1
,
PANAX-GINSENG
,
CROSS-LINKING
,
SCAFFOLD
,
TISSUE
,
CELLS
,
DRUG
,
DIFFERENTIATION
URI
https://hdl.handle.net/11511/96444
Journal
CARBOHYDRATE POLYMERS
DOI
https://doi.org/10.1016/j.carbpol.2021.118976
Collections
Department of Engineering Sciences, Article
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D. Atila et al., “In vitro evaluation of injectable Tideglusib-loaded hyaluronic acid hydrogels incorporated with Rg1-loaded chitosan microspheres for vital pulp regeneration,”
CARBOHYDRATE POLYMERS
, vol. 278, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/96444.