Determination of doxorubicin amount conjugated to mPEG-b-PCL copolymer via pH sensitive hydrazone bond pH duyarlı hidrazon bağıyla mPEG-b-PCL kopolimerine konjuge edilen doksorubisin miktarının belirlenmesi

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Date

2022-1-01

Author

Işik, Gülhan
Tezcaner, Ayşen
Hasırcı, Nesrin
Kızıltay, Aysel

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Turk Hijyen ve Deneysel Biyoloji Dergisi. All Rights Reserved.Objective: The aim of this study was to find the efficient medium to bind an anticancer drug, Doxorubicin (DOX), to a synthesized polymer, methoxy poly(ethylene glycol)-block-polycaprolactone (mPEG-b-PCL) via pH sensitive hydrazone bonds and to determine the amount of conjugated drug. Methods: DOX conjugation was carried out in two different media [dimethyl sulfoxide (DMSO) and methanol-trifluoroacetic acid (MeOH-TFA)]. The amount of conjugated drug was determined with two different methods. One method was applied the dissolution of the conjugate in chloroform: methanol (Ch: MeOH, 1: 1 v/v) solution without considering pH responsiveness, and the other method was after breaking pH sensitive hydrazone bonds in acidic medium [using three different media as 0.1 M hydrochloric acid (HCl), concentrated HCl (12 M HCl) and concentrated sulfuric acid (18.3 M H2SO4)]. Results: The highest conjugation efficiency was obtained when the conjugation was achieved in MeOHTFA solution, and for the polymer-drug conjugates after the treatment with concentrated sulfuric acid. Conclusion: It was concluded that, MeOH-TFA method was a good method for conjugation of DOX to mPEG-b-PCL copolymer, and H2SO4 (18.3 M) method was better than any other present in literature for determination of the amount of DOX linked to the polymer via hydrazone conjugation.

Subject Keywords

Doxorubicin, Drug conjugation, Hydrazine bond, Mpegb-pcl, Trinitrobenzene sulfonic acid

URI

https://hdl.handle.net/11511/101799

Journal

Turk Hijyen ve Deneysel Biyoloji Dergisi

DOI

https://doi.org/10.5505/turkhijyen.2022.04317

Collections

Department of Engineering Sciences, Article

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Citation Formats

G. Işik, A. Tezcaner, N. Hasırcı, and A. Kızıltay, “Determination of doxorubicin amount conjugated to mPEG-b-PCL copolymer via pH sensitive hydrazone bond pH duyarlı hidrazon bağıyla mPEG-b-PCL kopolimerine konjuge edilen doksorubisin miktarının belirlenmesi,” Turk Hijyen ve Deneysel Biyoloji Dergisi, vol. 79, no. 2, pp. 279–292, 2022, Accessed: 00, 2023. [Online]. Available: https://hdl.handle.net/11511/101799.