COMPARATIVE ANALYSIS OF BRAIN CELL CULTURES AND TISSUES IN ALZHEIMER’S DISEASE BASED ON DIFFERENTIAL EXPRESSION AND GENE SET ENRICHMENT

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2023-1-25

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Burduroğlu, Hüseyin Cahit

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Alzheimer’s disease is currently the most common cause of dementia in the world. It is a neurodegenerative disease that is diagnosed neuropathologically by observing B-amyloid plaques and neurofibrillary tangles in the brain. Transcriptional differentiations, protein regulations, and the interactions in between have been investigated by recent studies to understand from which brain cell type the disease stems, such as microglia, astrocytes, and neurons. These studies are mostly performed on brain tissue samples that bring certain challenges. With the addition of novel gene editing methods in recent years, cell models are gaining popularity as an alternative to studying Alzheimer’s disease. Yet, due to the complicated nature of the disease, cell models may not be representative enough. This study aims to determine the representativeness of cell models, in contrast to brain tissue samples, by comparing the two groups by their differentially expressed genes and enriched cellular functions. For this purpose, 19 projects (10 tissues, 9 cells) were selected for analysis from NCBI BioProject, which were then grouped by sex and cell types. The comparisons were made using the number of genes in gene sets that enrich certain biological processes. Distance matrices and topology plots were used for visualization.

Subject Keywords

Alzheimer's, transcriptomics, meta-analysis, differential expression, gene set enrichment, cell models

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https://hdl.handle.net/11511/102037

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Graduate School of Informatics, Thesis

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Citation Formats

H. C. Burduroğlu, “COMPARATIVE ANALYSIS OF BRAIN CELL CULTURES AND TISSUES IN ALZHEIMER’S DISEASE BASED ON DIFFERENTIAL EXPRESSION AND GENE SET ENRICHMENT,” M.S. - Master of Science, Middle East Technical University, 2023.