Date

2023-1-26

Author

Demir, Mehmet Alper

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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White blood cells (WBCs) play a critical role in fighting infections. An increase in the WBC level can indicate the presence of an infection, and the ratio of neutrophils to lymphocytes can provide insight into the type of infection. A higher neutrophil to lymphocyte count ratio suggests a bacterial infection. Accurate identification of the infection's origin is important to ensure proper treatment and avoid contributing to the growing issue of antibiotic resistance. A need for a system that offers accessibility and fast results exists in this field. Market systems deliver reliable results but may not be accessible due to their high costs. Instead, cell characterization based on electrical field response variations at different frequencies has been shown to be a alternative to available systems. This method depends on the characteristics of cells, such as cell membrane and cytoplasm. In this thesis, using the impedance flow cytometry technique, a microfluidic cell counting chip that determines the neutrophil / lymphocyte ratio in a sample is designed and characterized. Enumaration and differentiation between two cell groups is achieved. Experiment results give a promising outlook for developing of a cost-effective cell counter system that provides fast results.

Subject Keywords

Impedance flow cytometry, Cell counting

URI

https://hdl.handle.net/11511/102042

Collections

Graduate School of Natural and Applied Sciences, Thesis