Development of impedance flow cytometry system for white blood cell count

Demir, Mehmet Alper
White blood cells (WBCs) play a critical role in fighting infections. An increase in the WBC level can indicate the presence of an infection, and the ratio of neutrophils to lymphocytes can provide insight into the type of infection. A higher neutrophil to lymphocyte count ratio suggests a bacterial infection. Accurate identification of the infection's origin is important to ensure proper treatment and avoid contributing to the growing issue of antibiotic resistance. A need for a system that offers accessibility and fast results exists in this field. Market systems deliver reliable results but may not be accessible due to their high costs. Instead, cell characterization based on electrical field response variations at different frequencies has been shown to be a alternative to available systems. This method depends on the characteristics of cells, such as cell membrane and cytoplasm. In this thesis, using the impedance flow cytometry technique, a microfluidic cell counting chip that determines the neutrophil / lymphocyte ratio in a sample is designed and characterized. Enumaration and differentiation between two cell groups is achieved. Experiment results give a promising outlook for developing of a cost-effective cell counter system that provides fast results.


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In order to prevent, diagnose and treat vascular disease, detailed knowledge of blood blow and the response of blood vessels is essential. The use of computer simulations can provide researchers extremely useful tool of the interpretation and analysis of the circulatory sysytem in both physiological and pathological situations. In this work, we present a 2D mathematical model to forecast for some cardivascular diseases such as stenosis and aneurysms geometry. We consider a blood flow based on Stokes and Nav...
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This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100% selectivity at a flow rate of...
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Detection of circulating tumor cells (CTCs) from the bloodstream has a critical role in diagnosing and treatment of cancer. However, the number of CTCs in blood compared to other blood cells are extremely rare. In this thesis, various surface modifications strategies for detection of CTCs are studied in order to be used in the microfluidic detection systems. Functionalizing the gold surface with Self Assembled Monolayers (SAMs) used for attaching the EpCAM antibodies, which made possible to immobilize EpCAM...
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Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...
Citation Formats
M. A. Demir, “Development of impedance flow cytometry system for white blood cell count,” M.S. - Master of Science, Middle East Technical University, 2023.