Characterization and identification of human mesenchymal stem cells at molecular level

Aksoy, Ceren
Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FTIR microspectroscopy. In the first part of this study, global structural and compositional changes in BM-MSCs during beta thallasemia major (-TM) were investigated. The significant increase in lipid, protein, glycogen and nucleic acid concentrations in thalassemic MSCs with respect to healthy MSCs were attributed to enhanced cell proliferation and BM activity during ineffective erytropoiesis (IE). MTT assay results reflected increase in cellular activity of thallasemic BM-MSCs. The significant decreases in the concentrations of the mentioned macromolecules after BM transplantation therapy were interpreted as recovery of IE. Based on these changes, sampling groups were successfully discriminated by using cluster analysis. In the second part of this study, it was aimed to identify new molecular marker(s) in order to determine the effects of donor age on healthy BM-MSCs. The spectral results reflected that there were significant increases in the concentrations of saturated lipids, proteins, glycogen and nucleic acids in children and adolescent group BM-MSCs when compared with the infants, early and mid adults. These results were interpreted as increased proliferation activity in younger BM-MSCs. The results of MTT assay clarified the increased cellular activity. Spectral data of five different sampling groups was discriminated by hierarchical cluster analysis. The FTIR microspectroscopic imaging study was also performed in two different parts of the study in order to support the ATR-FTIR spectroscopy results. The FTIR microspectroscopic results were found in agreement with ATR-FTIR spectroscopy results.


Role of Vibrational Spectroscopy in Stem Cell Research
Aksoy, Ceren; Severcan, Feride (2012-01-01)
Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the ste...
Influence of micropatterns on human mesenchymal stem cell fate /
Hastürk, Onur; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Biotechnology (2016)
Mesenchymal stem cells (MSCs) are promising cell sources for tissue engineering applications as they can differentiate into a variety of adult cells types including osteoblasts. In vivo microenvironment of stem cells is known to provide both biochemical signals and micro- and nanoscale physical cues that influence the behavior and fate of stem cells. The use of soluble chemical factors is the most common strategy to guide the commitment of MSCs to specific lineages, but it is a cause of concern such as unsa...
Evaluating Oxygen Tensions Related to Bone Marrow and Matrix for MSC Differentiation in 2D and 3D Biomimetic Lamellar Scaffolds
Sayin, Esen; Baran, Erkan Turker; Elsheikh, Ahmed; Mudera, Vivek; Cheema, Umber; Hasırcı, Vasıf Nejat (2021-04-01)
The physiological O-2 microenvironment of mesenchymal stem cells (MSCs) and osteoblasts and the dimensionality of a substrate are known to be important in regulating cell phenotype and function. By providing the physiologically normoxic environments of bone marrow (5%) and matrix (12%), we assessed their potential to maintain stemness, induce osteogenic differentiation, and enhance the material properties in the micropatterned collagen/silk fibroin scaffolds that were produced in 2D or 3D. Expression of ost...
Differentiation of BMSCs into Nerve Precursor Cells on Fiber-Foam Constructs for Peripheral Nerve Tissue Engineering
Dursun Usal, Tuğba; YÜCEL, DENİZ; Hasırcı, Vasıf Nejat (2018-06-01)
Bone marrow stem cells (BMSCs) are frequently used in nerve tissue engineering studies due to ease of their isolation and high potential for differentiation into nerve cells. A bilayer fiber-foam construct containing nanofibrous elements to house and guide BMSCs was designed as a model to study the regeneration of damaged peripheral nerve tissue and eventually serve as a nerve guide. The construct consisted of a) a macroporous bottom layer to serve as the backing and support, and for nutrient transport, and...
Effect of double growth factor release on cartilage tissue engineering
Ertan, Ayse Burcu; Yilgor, Pinar; Bayyurt, Banu; Calikoglu, Ayse Ceren; Kaspar, Cigdem; Kök, Fatma Neşe; KÖSE, GAMZE; Hasırcı, Vasıf Nejat (2013-02-01)
The effects of double release of insulin-like growth factor I (IGF-I) and growth factor 1 (TGF1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF1, respectively. On tissue culture polystyrene (TCPS), TGF-1 released from PNIPAM nanoparticles was found to ...
Citation Formats
C. Aksoy, “Characterization and identification of human mesenchymal stem cells at molecular level,” Ph.D. - Doctoral Program, Middle East Technical University, 2012.