Spheroid Engineering in Microfluidic Devices

Date

2023-01-01

Author

Tevlek, Atakan
Kecili, Seren
Ozcelik, Ozge S.
Külah, Haluk
Tekin, H. Cumhur

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Two-dimensional (2D) cell culture techniques are commonly employed to investigate biophysical and biochemical cellular responses. However, these culture methods, having monolayer cells, lack cell-cell and cell-extracellular matrix interactions, mimicking the cell microenvironment and multicellular organization. Three-dimensional (3D) cell culture methods enable equal transportation of nutrients, gas, and growth factors among cells and their microenvironment. Therefore, 3D cultures show similar cell proliferation, apoptosis, and differentiation properties to in vivo. A spheroid is defined as self-assembled 3D cell aggregates, and it closely mimics a cell microenvironment in vitro thanks to cell-cell/matrix interactions, which enables its use in several important applications in medical and clinical research. To fabricate a spheroid, conventional methods such as liquid overlay, hanging drop, and so forth are available. However, these labor-intensive methods result in low-throughput fabrication and uncontrollable spheroid sizes. On the other hand, microfluidic methods enable inexpensive and rapid fabrication of spheroids with high precision. Furthermore, fabricated spheroids can also be cultured in microfluidic devices for controllable cell perfusion, simulation of fluid shear effects, and mimicking of the microenvironment-like in vivo conditions. This review focuses on recent microfluidic spheroid fabrication techniques and also organ-on-a-chip applications of spheroids, which are used in different disease modeling and drug development studies.

URI

https://hdl.handle.net/11511/102707

Journal

ACS OMEGA

DOI

https://doi.org/10.1021/acsomega.2c06052

Collections

Department of Electrical and Electronics Engineering, Article

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Citation Formats

A. Tevlek, S. Kecili, O. S. Ozcelik, H. Külah, and H. C. Tekin, “Spheroid Engineering in Microfluidic Devices,” ACS OMEGA, vol. 8, no. 4, pp. 3630–3649, 2023, Accessed: 00, 2023. [Online]. Available: https://hdl.handle.net/11511/102707.