FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media

Date

1999-09-15

Author

Haris, PI
Severcan, Feride

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With increasing use of proteins in many different applications, ranging from phramaceuticals to biosensors and biomaterials, there has emerged a need for protein structural characterisation in diverse environments. In many cases it is not sufficient to just have the three-dimensional structure of a protein in H2O or in the crystalline state. Often information on the structural properties of a protein is required in the presence of organic solvents, detergent micelles, phospholipid membranes and so on. Fourier transform infrared spectroscopy (FTIR) has been identified as one of the few techniques that can be applied for structural characterisation of proteins in such environments. Here we discuss how this technique is being used to obtain information on protein structure and stability in both aqueous and non-aqueous media. Examples are drawn from our studies of water soluble proteins and membrane proteins.

Subject Keywords

Infrared spectroscopy, Membrane proteins, Protein secondary structure, Protein folding, Protein stability, Protein conformation, FTIR spectroscopy

URI

https://hdl.handle.net/11511/56768

Journal

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

DOI

https://doi.org/10.1016/s1381-1177(99)00030-2

Collections

Department of Biology, Article

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Citation Formats

P. Haris and F. Severcan, “FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media,” JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, pp. 207–221, 1999, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56768.