Studying Tunicata WBR Using Botrylloides anceps

Date

2022-04-01

Author

Karahan, Arzu
Öztürk, Esra
Temiz, Berivan
Blanchoud, Simon

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Tunicates are marine filter-feeding invertebrates that can be found worldwide and which are the closest phylogenetic group to the vertebrates (Craniata). Of particular interest, colonial tunicates are the only known chordates that can undergo Whole-Body Regeneration (WBR) via vascular budding. In Botrylloides anceps, a fully functional adult regenerates from a fragment of the vascular system in around 2 weeks after amputation. In this chapter, we present protocols to collect B. anceps colonies, confirm their species, breed them in the lab, monitor WBR and perform histological staining on cryosections.

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https://hdl.handle.net/11511/107275

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Graduate School of Marine Sciences, Book / Book chapter