Tissue engineered retinal pigment epithelium

Tezcaner, Ayşen


Interdependence of pulsed ultrasound and shear stress effects on cell morphology and gene expression
Mccormick, Susan M.; Saini, Vikas; Yazıcıoğlu, Yiğit; Demou, Zoe N.; Royston, Thomas J. (Springer Science and Business Media LLC, 2006-03-01)
Fluid shear stress is a key biomechanical regulatory factor in a several biological systems including bone tissue. Bone cells are also regulated by exogenous acoustic vibration, which has therapeutic benefits. In this study, we determined the effects of shear stress and pulsed ultrasound (US), alone and in series on osteoblast morphology and gene expression. We observed that shear stress (19 dyne/cm(2)) elongated SaOS-2 cells at 3, 6, 24, and 48 h decreasing their shape index from control values of 0.51 +/-...
Tissue engineered cartilage on collagen and PHBV matrices
Kose, GT; Korkusuz, F; Ozkul, A; Soysal, Y; Ozdemir, T; Yildiz, C; Hasırcı, Vasıf Nejat (2005-09-01)
Cartilage engineering is a very novel approach to tissue repair through use of implants. Matrices of collagen containing calcium phosphate (CaP-Gelfix (R)), and matrices of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) were produced to create a cartilage via tissue engineering. The matrices were characterized by scanning electron microscopy (SEM) and electron diffraction spectroscopy (EDS). Porosity and void volume analysis were carried out to characterize the matrices. Chondrocytes were isola...
Bone tissue generation on biodegradable polymeric scaffolds
Torun Köse, Gamze; Hasırcı, Vasıf Nejat; Korkusuz, Feza; Department of Biotechnology (2002)
In the present study, tissue engineered bone was produced on calcium phosphate loaded collagen and PHBV8 matrices. Osteoblasts isolated from rat bone marrow were characterized by light microscopy, alkaline phosphatase (ALP) activity, osteocalcin determination assay, and Western blots for integrin. Population doubling time of the cells was found as 50 ± 2 h at 37 °C in a CO2 incubator. PHBV8 foams iiiwere treated with rf-oxygen plasma to modify their surface chemistry and hydrophilicity to increase the reatt...
Biodegradable poly (ester-urethane) scaffolds for bone tissue engineering
Kızıltay, Aysel; Hasırcı, Nesrin; Department of Biotechnology (2011)
During last decade, polyurethanes (PUs) which are able to degrade into harmless molecules upon implantation have received a significant level of attention as a biomaterial in tissue engineering applications. Many studies are focused especially on development of PUs based on amino acid derivatives; however, there are only few applications of amino acid based PUs in tissue engineering. In this study, a biocompatible and biodegradable thermoplastic poly(ester-urethane) (PEU) based on L-lysine diisocyanate (LDI...
Preparation and characterization of polymeric hard tissue supports
Endoğan, Tuğba; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat; Department of Polymer Science and Technology (2013)
In recent years, the demand for the use of artificial materials have increased drastically in biomedical field since autografts have limited availability and allografts lead to immune response problems. The aim of this thesis was to prepare polymer based materials which will be used for either supportive or regenerative materials for hard tissue applications. In the first part of the thesis, hydroxyapatite (HAp) containing acrylic based bone cements as supportive materials were prepared. In this thesis, bon...
Citation Formats
A. Tezcaner, “Tissue engineered retinal pigment epithelium,” Ph.D. - Doctoral Program, Middle East Technical University, 2001.