Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Biodegradable poly (ester-urethane) scaffolds for bone tissue engineering
Download
index.pdf
Date
2011
Author
Kızıltay, Aysel
Metadata
Show full item record
Item Usage Stats
287
views
117
downloads
Cite This
During last decade, polyurethanes (PUs) which are able to degrade into harmless molecules upon implantation have received a significant level of attention as a biomaterial in tissue engineering applications. Many studies are focused especially on development of PUs based on amino acid derivatives; however, there are only few applications of amino acid based PUs in tissue engineering. In this study, a biocompatible and biodegradable thermoplastic poly(ester-urethane) (PEU) based on L-lysine diisocyanate (LDI) and polycaprolactone diol (PCL) was synthesized and used for the preparation of two dimensional (2D) films and three dimensional (3D) scaffolds. The resulting polymer was casted as 2D films for full characterization purpose and it was found that it is highly elastic with modulus of elasticity ~12 MPa. Surfaces of 2Ds were modified via micropatterning and fibrinogen coating to check the material-cell interaction. The 3D scaffolds were obtained by salt leaching and rapid prototyping (bioplotting) techniques. The 3D scaffolds had various pore size and porosity with different mechanical strength. The bioplotted scaffolds had uniform pore size of ~450 µm and exhibited higher compressive modulus (~4.7 MPa) compared to those obtained by salt leaching (~147 kPa). Salt leached 3D scaffolds had inhomogenous pore size distribution in the range of 5 µm - 350 µm and demonstrated greatest degradation profile compared to 2D films and 3D bioplotted samples under enzymatic condition. Rat bone marrow stem cells (BMSCs) were used to investigate the biocompatibility of the polymer and suitability of fabricated scaffolds for osteogenesis. Presence of micropatterns on 2D matrices did not show any influence on osteoblastic function, but presence of fibrinogen enhanced cell attachment and proliferation. All of the fabricated 3D PEU matrices supported proliferation, osteoblastic differentiation and extracellular matrix (ECM) deposition with highest osteoblastic activity on bioplotted scaffolds which confirmed by von Kossa staining and EDX analysis. The results indicated that the synthesized PEU based scaffolds were able to induce osteoblastic differentiation and mineralization of BMSC and therefore these scaffolds can be good candidates to be used in bone tissue engineering
Subject Keywords
Tissue engineering.
URI
http://etd.lib.metu.edu.tr/upload/12613811/index.pdf
https://hdl.handle.net/11511/20912
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Free standing layer-by-layer films of polyethyleneimine and poly(l-lysine) for potential use in corneal stroma engineering
Altay, Gizem; Hasırcı, Vasıf Nejat; Khademhosseini, Ali; Department of Biomedical Engineering (2011)
In this study we fabricated free standing multilayer films of polyelectrolyte complexes for potential use in tissue engineering of corneal stroma by using the layer-by-layer (LbL) approach. In the formation of these LbL films negatively charged, photocrosslinkable (methacrylated) hyaluronic acid (MA-HA) was used along with polycations polyethyleneimine (PEI) and poly(L-lysine) (PLL). Type I collagen (Col) was blended in with PLL for improving the water absorption and cell attachment properties of the films....
Polymeric scaffolds for bioactive agent delivery in bone tissue engineering
Uçar, Şeniz; Hasırcı, Nesrin; Yılgör, Pınar; Department of Chemistry (2012)
Tissue engineering is a multidisciplinary field that is rapidly emerging as a promising new approach in the restoration and reconstruction of tissues. In this approach, three dimensional (3D) scaffolds are of great importance. Scaffolds function both as supports for cell growth and depot for sustained release of required active agents (e.g. enzymes, genes, antibiotics, growth factors). Scaffolds should possess certain properties in accordance with usage conditions. Wet-spinning is a simple technique that ha...
Cellulose-based electrospun scaffolds for tissue engineering applications
Atila, Deniz Hazal; Tezcaner, Ayşen; Yazgan Karataş, Ayten; Department of Engineering Sciences (2014)
With the use of a scaffold as support material, adequate number of cells, and bioactive molecules, tissue engineering applications intend to promote the regeneration of tissues or to replace failing or malfunctioning tissues/organs. In this study, electrospun 2D and 3D cellulose-based scaffolds were aimed to be produced with pullulan (PULL). Cellulose acetate (CA) and PULL powders in various ratios (80/20, 50/50, and 80/20) were dissolved in DMAc/DMSO solvent system and electrospun as either 2D or 3D forms....
Wet spun PCL scaffolds for tissue engineering
Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Fabrication and characterization of bilayered tissue scaffolds incorporating bioactive agents for skin tissue engineering applications
Aktürk, Ömer; Keskin, Dilek; Bilici, Temel; Department of Engineering Sciences (2015)
In this study, it was aimed to fabricate tissue scaffolds from different biological polymers (collagen, silk fibroin and sericin) for skin tissue engineering applications. For this purpose, bilayered scaffolds composed of epidermal (collagen/sericin films) and dermal (collagen sponges, collagen matrices or silk fibroin matrices) layers were produced with different biomaterial fabrication methods. Casting and solvent evaporation (film), lyophilization/freeze-drying (sponge) and dry/wet electro-spinning (micr...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
A. Kızıltay, “Biodegradable poly (ester-urethane) scaffolds for bone tissue engineering,” Ph.D. - Doctoral Program, Middle East Technical University, 2011.
