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Layer-by-layer assembly of chitosan/alginate thin films containing Salmonella enterica bacteriophages for antibacterial applications
Date
2024-03-15
Author
Tidim, Gökçe
Guzel, Mustafa
Soyer, Yeşim
Erel Göktepe, İrem
Metadata
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The emergence of antibiotic resistant bacteria and the ineffectiveness of routine treatments inspired development of alternatives to biocides for antibacterial applications. Bacteriophages are natural predators of bacteria and are promising alternatives to antibiotics. This study presents fabrication of a Salmonella enterica bacteriophage containing ultra-thin multilayer film composed of chitosan and alginate and demonstrates its potential as an antibacterial coating for food packaging applications. Chitosan/alginate film was prepared through layer-by-layer (LbL) self-assembly technique. A bacteriophage, which belongs to Siphoviridae morphotype (MET P1-001_43) and infects Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Enteritidis), was post-loaded into chitosan/alginate film. The LbL growth, stability, and surface morphology of chitosan/alginate film as well as phage deposition into multilayers were analysed through ellipsometry, QCM-D and AFM techniques. The bacteriophage containing multilayers showed antibacterial activity at pH 7.0. In contrast, anti-bacterial activity was not observed at acidic conditions. We showed that wrapping a Salmonella Enteritidis contaminated chicken piece with aluminium foil whose surface was modified with phage loaded chitosan/alginate multilayers decreased the number of colonies on the chicken meat, and it was as effective as treating the meat directly with phage solution.
Subject Keywords
Alginate
,
Antibacterial
,
Bacteriophage
,
Chitosan
,
Food packaging
,
Layer-by-layer self-assembly
URI
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85181767583&origin=inward
https://hdl.handle.net/11511/108093
Journal
Carbohydrate Polymers
DOI
https://doi.org/10.1016/j.carbpol.2023.121710
Collections
Department of Chemistry, Article
Citation Formats
IEEE
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MLA
BibTeX
G. Tidim, M. Guzel, Y. Soyer, and İ. Erel Göktepe, “Layer-by-layer assembly of chitosan/alginate thin films containing Salmonella enterica bacteriophages for antibacterial applications,”
Carbohydrate Polymers
, vol. 328, pp. 0–0, 2024, Accessed: 00, 2024. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85181767583&origin=inward.