Investigation of polyhydroxybutyrate (PHB) production by rhodobacter sphaeroides O.U.001

Suludere, Demet


Investigation of bioprocess parameters for glucose isomerase production by Bacillus thermoantarcticus
Haykır, Işık; Çalık, Pınar; Department of Chemical Engineering (2007)
In this study, the effects of bioprocess parameters on glucose isomerase roduction by Bacillus thermoantarcticus (DSMZ 9572) were investigated. For his purpose, firstly, in laboratory scale shake-bioreactors, a defined medium was designed in terms of its carbon and nitrogen sources, to achieve the highest glucose isomerase activity. Optimum concentrations of medium components were determined by a statistical approach, namely Response Surface Methodology (RSM). RSM defined the relationship between the respon...
Hoşafcı, Ertan; Bayramoğlu, Tuba Hande; Koku, Harun; Department of Environmental Engineering (2023-1-26)
The aim of this thesis study is to investigate factors affecting hydrogen (H2) and poly-hydroxybutyrate (PHB) production via photofermentation using two strains of Rhodobacter capsulatus species. To this purpose, three experimental designs were set up, namely Set 1, Set 2 and Set 3. In Set 1, various approaches in pretreatment of bacterial biomass were investigated. For R. capsulatus Wild Type (WT) strain, employment of different pretreatment methods did not make a significant difference, where an average ...
Investigation of sugar metabolism in rhizopus oryzae
Büyükkileci, Ali Oğuz; Hamamcı, Haluk; Department of Biotechnology (2007)
Rhizopus oryzae is a filamentous fungus, which can produce high amounts of L(+)-lactic acid and produces ethanol as the main by-product. In an effort to understand the pyruvate branch point of this organism, fermentations under different inoculum and glucose concentrations were carried out. At low inoculum size (1x103 spores ml-1), high amount of lactate (78 g l-1) was produced, whereas high ethanol concentration (37 g l-1) was obtained at high inoculum sizes (1x106 spores ml-1). Decreasing working volume i...
Investigation of bioreactor operation conditions for serine alkaline protease production by Bacillus subtilis
Özdemir, Elif; Çalık, Pınar; Department of Chemical Engineering (2003)
In this study, complex medium was designed for increasing serine alkaline protease (SAP) production by recombinant Bacillus sp. In this context, the effects of glucose, sucrose, inorganic nitrogen source, casein, defatted soybean, and molasses and their concerted effects were investigated in agitation (N=200 min"1) and heating rate (T=37°C) controlled laboratory scale bioreactors. For this purpose, experiments were started by microorganism selection. It was found that, most suitable microorganism for comple...
Investigation of thermostable recombinant glucose isomerase production by sucrose utilizing escherichia coli
Akdağ, Burcu; Çalık, Pınar; Özdamar, Tunçer H.; Department of Biotechnology (2013)
The aim of this M.Sc. thesis is to investigate the production of thermostable glucose isomerase (GI; EC by metabolically engineered sucrose-utilizing Escherichia coli in the designed molasses-based production media. Throughout the experiments the cell growth, sucrose consumption, recombinant GI activity, and by-product concentrations were analyzed. For this purpose, in the first part of this thesis, pRSETA plasmid carrying the thermostable GI encoding gene from Thermus thermophilus (xylA) was isola...
Citation Formats
D. Suludere, “Investigation of polyhydroxybutyrate (PHB) production by rhodobacter sphaeroides O.U.001,” Middle East Technical University, 2001.