Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Glutamine withdrawal leads to the preferential activation of lipid metabolism in metastatic colorectal cancer
Download
Glutamine withdrawal leads to the preferential.pdf
Date
2024-10-01
Author
Güleç Taşkıran, Aliye Ezgi
Karaoğlu, Diren Arda
EYLEM, CEMİL CAN
Ermiş, Çağdaş
Güderer, İsmail
NEMUTLU, EMİRHAN
DEMİRKOL CANLI, SEÇİL
Banerjee, Sreeparna
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
39
views
4
downloads
Cite This
Introduction: Glutamine is a non-essential amino acid that is critical for cell growth. However, the differential metabolism of L-glutamine in metastatic versus primary colorectal cancer (CRC) has not been evaluated adequately. Materials and methods: Differential expression of glutamine-related genes was determined in primary versus metastatic CRC. Univariate Cox regression and hierarchical clustering were used to generate a gene signature for prognostication. Untargeted metabolomics and 18O based fluxomics were used to identify differential metabolite levels and energy turnover in the paired primary (SW480) and metastatic (SW620) CRC cells. Western blot and qRT-PCR were used to validate differential gene expression. Subcellular localization of E-cadherin was determined by immunocytochemistry. Lipid droplets were visualized with Nile Red. Results: The GO term “Glutamine metabolism” was significantly enriched in metastatic versus primary tumors. Supporting this, SW620 cells showed decreased membrane localization of E-cadherin and increased motility upon L-Glutamine withdrawal. A glutamine related signature associated with worse prognosis was identified and validated in multiple datasets. A fluxomics assay revealed a slower TCA cycle in SW480 and SW620 cells upon L-Glutamine withdrawal. SW620 cells, however, could maintain high ATP levels. Untargeted metabolomics indicated the preferential metabolism of fatty acids in SW620 but not SW480 cells. Lipids were mainly obtained from the environment rather than by de novo synthesis. Conclusions: Metastatic CRC cells can display aberrant glutamine metabolism. We show for the first time that upon L-glutamine withdrawal, SW620 (but not SW480) cells were metabolically plastic and could metabolize lipids for survival and cellular motility.
Subject Keywords
Colorectal cancer
,
L-Glutamine
,
Lipid metabolism
,
Metabolic plasticity
URI
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85201377055&origin=inward
https://hdl.handle.net/11511/111216
Journal
Translational Oncology
DOI
https://doi.org/10.1016/j.tranon.2024.102078
Collections
Department of Biology, Article
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
A. E. Güleç Taşkıran et al., “Glutamine withdrawal leads to the preferential activation of lipid metabolism in metastatic colorectal cancer,”
Translational Oncology
, vol. 48, pp. 0–0, 2024, Accessed: 00, 2024. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85201377055&origin=inward.