Salmonella'ya Karşı Faj Endolizinleri

Date

2023-07-19

Author

Yetişkin, Segah
Yücefaydalı, Ayşenur
Soyer, Yeşim

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Phage endolysins againstSalmonellaSegâh Yetiskin1, Aysenur Yucefaydali1, Yeşim Soyer1,2*1Department of Food Engineering, Middle East Technical University. Ankara, Turkey.2Department of Biotechnology, Middle East Technical University. Ankara, TurkeySalmonellais one of the major bacteria causing foodborne diseases in the world.Salmonellais gram negative, non-spore forming and rod shape bacteria, and those with enteric fever and non-typhoidal salmonellosis cause gastroenteritis problems. Bacteriophages are the viruses that infect bacterial cells. Endolysins, bacteriophage lytic enzymes, are enzymes produced during growth of bacteriophage in the cell. Endolysins cause cell death by disturbing cell wall and cell membrane of the bacteria and to release new forming viruses. Lysins aim important and conserved regions of the cell which allow to release new viruses. Because of these features, resistance ability of bacteria to endolysins is less than to antibiotics. Since eukaryotes don’t have peptidoglycan, endolysins doesn’t affect human and animals. In this study, bacteriophage, named P1-137, was isolated and characterized and its endolysin was expressed. The genome of the phage was analyzed and genes producing endolysins were selected. Endolysin gene was transferred toE. coliBL21 strain using vector and production was achieved. After production, purification was done and the effects of these proteins on bacteria were investigated.

URI

https://hdl.handle.net/11511/112007

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Department of Food Engineering, Conference / Seminar