Optimization of Regeneration and Agrobacterium mediated transformation of wheat (Triticum aestivum L.cv. Yüregir 89)

Download

index.pdf

Date

2004

Author

Demirbaş, Didem

Metadata

Show full item record

Item Usage Stats

109
views

54
downloads

The objective of this study was to optimize regeneration parameters of immature inflorescence culture of Triticum aestivum cv. Yüregir-89. The effects of dark incubation period and explant region on regeneration success were tested. Immature inflorescences were cut into 3 pieces as tip, mid, base and put onto 2mg /L 2,4-dichlorophenoxyacetic acid containing callus induction medium. These explants were taken to regeneration after 6, 9, 13 weeks of dark incubation period. The regeneration capacities of calli were determined as rooting and shooting percentages. Shooting percentages were found to be 72.0 % for 6 weeks of dark incubation and 64.1 % for 9 weeks of dark incubation while it decreases to 26.1 % in 13 weeks of dark incubation period. This showed that prolonged dark incubation period decreased regeneration capacity of the callus. There was no significant difference in regeneration capacities of tip, mid and base regions of immature inflorescences, which reveals the potential of every region of inflorescence to be used as explant source in further transformation studies. Besides regeneration studies, optimization of transformation parameters for Turkish wheat cultivar Yüregir by using Agrobacterium tumefaciens AGLI containing binary vector pALl56 was performed. Transformation efficiencies were determined by monitoring the transient expression of uidA gene via histochemical GUS assay. Three to four weeks old calli were found to be more responsive to Agrobacterium-mediated transformation. Different media were tested for utilization during co-cultivation period. It was found that including phenolic compound acetosyringone along with ascorbic acid as an antioxidant was essential for succesful transformation.

Subject Keywords

Food Crops.

URI

http://etd.lib.metu.edu.tr/upload/3/12605454/index.pdf
https://hdl.handle.net/11511/14379

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Optimization of tissue culture, generation and agrobacterium mediated transformation parameters in winter wheat cultivars (Kızıltan-91 and Bezostaja-01) Kavas, Musa; Yücel, Ayşe Meral; Department of Biology (2005) The objective of this study was to optimize tissue culture and regeneration parameters of immature inflorescence culture of Triticum aestivum cv. Bezostaja- 01 and Triticum durum cv. Kızıltan-91. The effects of callus age and vernalisation time of explants on regeneration success were evaluated. For determination of optimum vernalisation time of immature inflorescence, plants subjected to 4°C for 1, 2, 3, 4, and 5 weeks, respectively. Tillers containing immature inflorescences were collected at the same tim...
Campanian-Maastrichtian planktonic foraminiferal investigation and biostratigraphy (Kokaksu section, Bartın, NW Anatolia) : remarks on the cretaceous paleoceanography based on quantitative data Güray, Alev; Altıner, Sevinç; Department of Geological Engineering (2006) The aim of this study is to delineate the Campanian-Maastrichtian boundary by using planktonic foraminifers. In this manner, Kokaksu Section (Bartın, NW Anatolia) was selected and the Akveren Formation, characterized by a calciturbiditic-clayey limestone and marl intercalation of Campanian-Maastrichtian age, was examined. 59 samples were emphasized for position of boundary. Late Campanian-Maastrichtian planktonic foraminifers were studied in thin section and by washed samples. Two different biostratigraphic...
Taxonomy and distribution of the benthic foraminifera in the gulf of İskenderun, Eastern Mediterranean Oflaz, Sabire Aslı; Altıner, Sevinç; Department of Geological Engineering (2006) The present study aims to investigate the foraminiferal assemblages of the recent samples in terms of abundance and diversity, to determine the bathymetrical and the geographical distributions of the foraminiferal assemblages. This study further intends to put forward the responses of foraminifers to environmental factors (e.g. bathymetry, salinity, substrate, pollution, water currents, etc.) on the distribution of foraminifers. In this manner, foraminiferal fauna has been taxonomically identified and quant...
The effects of hydrogen peroxide, gallic acid and resveratrol on growth and catalase production of aspergillus fumigatus Doğan, Tunca; Ögel, Zümrüt Begüm; Department of Food Engineering (2008) The aim of this study was to analyze the effect of hydrogen peroxide and selected phenolic compounds on growth and catalase production of Aspergillus fumigatus. As a result of growing A. fumigatus at different temperatures it was observed that, growth and catalase production of this species were highest at 37 °C. Catalase production was highest in the presence of 1 mM H2O2, yielding a significant 3 fold increase with respect to the control. Biomass was also increased by 1,44 fold with respect to the control...
Effect of high hydrostatic pressure treatment on some quality properties, squeezing pressure effect and shlf life pf pomegranate (Punica granatum) juice against thermal treatment Gültekin, Necmiye Büşra; Alpas, Hami; Department of Food Engineering (2012) The aim of this study was to investigate the effect of high hydrostatic pressure (HHP) treatment (200, 300, 400 MPa; 5⁰C, 15⁰C, 25⁰C; 5 and 10 minutes) on some quality properties of two different squeezing pressure processed pomegranate juices against traditional thermal treatment at 85⁰C/10 min. Among these combinations, for 100 psi squeezed, 400 MPa 15⁰C 5 min and for 150 psi, 400 MPa 5⁰C 10 min were chosen the best. Antioxidant activity, total phenolic content, total monomeric anthocyanin concentration, ...

Citation Formats

D. Demirbaş, “Optimization of Regeneration and Agrobacterium mediated transformation of wheat (Triticum aestivum L.cv. Yüregir 89),” M.S. - Master of Science, Middle East Technical University, 2004.