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Biochemical characterization of recombinant 20s proteasome from thermoplasma volcanium and cloning of it's regulatory subunit gene

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2006
Baydar, Gözde
In this study, we have characterized some biochemical and electrophoretic features of recombinant 20S Proteasome from a thermoacidophilic archaeon Thermoplasma volcanium. As revealed by SDS-PAGE the 20S Proteasome was composed of two subunits, ?- and β- subunits with estimated molecular masses of 24 kDa and 23 kDa, respectively. The highest chymotryptic activity was observed over an alkaline pH range (pH 8.0 ا pH 9.0) and the optimum temperature for the activity was determined as 85oC. The heat stability of proteasome was quite high after treatment at 98oC for 30 minutes, 64 % of the activity has still been retained. The highest activity associated with the Thermoplasma volcanium proteasome was found to be peptidylglutamyl peptidase activity. Within the scope of this project, also, we have cloned a 26S Proteasome related Regulatory Subunit gene of Thermoplasma volcanium. For cloning we have followed a PCR based approach. Amplification of 26S Proteasome Regulatory Subunit gene from chromosomal DNA of Tp. volcanium yielded a product of 1419 bp containing an open reading frame of 1128 bp comprising the structural gene. The PCR amplicon was cloned using pDrive vector in E.coli TG-1 cells. Out of ten putative recombinants, three plasmids, E.coli pD1-6, E.coli pD2-3, E.coli pD3-1, were proved to be true recombinants and selected for further characterization by restriction mapping and expression studies. ATPase activities of cell free extracts from both recombinant and non-recombinant E.coli strains were measured and found that ATPase activities in cell free extracts of recombinant strains were 10 times higher than non-recombinants. This result indicates sucessful expression of the cloned regulatory subunit gene with ATPase activity in E.coli.