Molecular identification and typing of lactobacillus delbrueckii subspecies bulgaricus and streptococcus thermophilus

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2008

Author

Cebeci Aydın, Aysun

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Lactic acid bacteria are associated with preservation of foods, including milk, meat and vegetables. Yoghurt is produced by the cooperative action of two starter bacteria; S. thermophilus and L. delbrueckii subsp. bulgaricus. In this study, identification and typing of yoghurt starter bacteria were aimed. Traditional home made yoghurts were collected from different areas of Turkey, identification of those isolates at species and subspecies level and typing at strain level were achieved using PCR based methods. In our study, identification of yogurt starter bacteria was studied using species specific primers and ARDRA. These methods were inefficient in identification of yoghurt starter bacteria, at species and subspecies level. Consequently, a reliable and quick method for accurate identification of yoghurt starter bacteria was developed. The new method focuses on amplification of methionine biosynthesis genes, for selective identification of yoghurt starter bacteria together with some cheese starters. Further discrimination by ARDRA enabled differentiation of yoghurt starter bacteria from cheese starters. Confirmation of the proposed method has been accomplished by partial sequencing of the 16S rRNA gene. After correct identification of starter bacteria had been achieved, the strains were typed at strain level using RAPD-PCR and MLST. RAPD-PCR with primer 1254 resulted better fingerprints, compared to primer M13 at strain level. Comparisons of the two typing methods showed that RAPD-PCR revealed strain diversity better than MLST, however MLST was a more robust and reliable method and resulted in clustering of the strains depending on the isolation source.

Subject Keywords

Biochemistry., Bacteria.

URI

http://etd.lib.metu.edu.tr/upload/3/12609333/index.pdf
https://hdl.handle.net/11511/17551

Collections

Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

A. Cebeci Aydın, “Molecular identification and typing of lactobacillus delbrueckii subspecies bulgaricus and streptococcus thermophilus,” Ph.D. - Doctoral Program, Middle East Technical University, 2008.