Causes and consequences of seasonal variation of phosphoglucomutase (pgm) enzyme polymorphism in honeybees, (apis mellifera l.) of turkey

Güldüren, Zerrin
Phosphoglucomutase (PGM) is one of the central enzymes in energy metabolism at a branch point at the head of the metabolic pathway leading into glycogen metabolism, pentose shunt and the main glycolytic cycle, catalyzing the reversible interconversion of glucose-1-phosphate to glucose-6-phosphate. Whole year, month to month analysis of pattern of allozyme variation at Pgm and Hk loci in Apis mellifera L. from three provinces; Kırklareli, Artvin, and Hatay revealed that there is significant seasonal variation of allozyme frequencies at Pgm locus (P<0.001). The difference in genotype frequencies between summer and winter samples is apparent in Pgm, whereas at Hk locus, which is analyzed as a control there is seasonal variation in genotype frequencies. Biochemical measurements of the enzyme activities and glycogen content of different Pgm genotypes were performed to determine the effect of different Pgm genotypes on the physiological performance of the honeybees and it was observed that both enzyme activity and glycogen amount is higher in heterozygote individuals which are in high frequency during winter months (P<0.0001). Furthermore, PGM enzyme activity and glycogen content was found to be significantly correlated. These findings clearly demonstrate that biochemical differences between different Pgm genotypes have functional correlates that lead to significant variations in glycogen content of the honeybees and may have adaptive consequences.


Molecular recognition of poly(A) by small ligands: an alternative method of analysis reveals nanomolar, cooperative and shape-selective binding
Persil Çetinkol, Özgül (Oxford University Press (OUP), 2009-02-01)
A few drug-like molecules have recently been found to bind poly(A) and induce a stable secondary structure (T(m)approximate to 60 degrees C), even though this RNA homopolymer is single-stranded in the absence of a ligand. Here, we report results from experiments specifically designed to explore the association of small molecules with poly(A). We demonstrate that coralyne, the first small molecule discovered to bind poly(dA), binds with unexpectedly high affinity (K(a) >10(7) M(-1)), and that the crescent sh...
Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters
SHIN, Ilchung; RAY, Judhajeet; Gupta, Vinayak; İlgü, Müslüm; Beasley, Jonathan; BENDICKSON, Lee; MEHANOVIC, Samir; Kraus, George A.; Nilsen-Hamilton, Marit (Oxford University Press (OUP), 2014-01-01)
We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that can be expressed from a promoter of choice. For imaging, the cells are incubated with their ligands that are separately conjugated with one of the FRET pair, Cy3 and Cy5. The IMAGEtags were expressed in yeast from ...
Expression of recombinant acid protease (thermopsin) gene from Thermoplasma volcanium
Koyuncu, Bilsev; Kocabıyık, Semra; Department of Biology (2006)
Acid proteases, commonly known as aspartic proteases are degredative enzymes which catalyze the cleavage reaction of peptide bonds in proteins with a pH optimum in the acidic range (pH 3-4). Acid proteases have crucial roles in metabolism. Moreover, they are used in different fields of industry. Thermophilic microorganisms, especially archaea, gain special interest because of their thermal stability for both fundamental and industrial researches. Thermopsin is an extracellular acid protease and a member of ...
Eicosanoid pathway in colorectal cancer: Recent updates
Tuncer, Sinem; Banerjee, Sreeparna (2015-11-07)
Enzymatic metabolism of the 20C polyunsaturated fatty acid (PUFA) arachidonic acid (AA) occurs via the cyclooxygenase (COX) and lipoxygenase (LOX) pathways, and leads to the production of various bioactive lipids termed eicosanoids. These eicosanoids have a variety of functions, including stimulation of homeostatic responses in the cardiovascular system, induction and resolution of inflammation, and modulation of immune responses against diseases associated with chronic inflammation, such as cancer. Because...
Metabolic flux analysis for serine alkaline protease fermentation by Bacillus licheniformis in a defined medium: Effects of the oxygen transfer rate
Çalık, Pınar; Ozdamar, TH (1999-07-01)
The metabolic fluxes through the central carbon pathways in the bioprocess for serine alkaline protease (SAP) production by Bacillus licheniformis were calculated by the metabolic flux-based stoichiometric model based on the proposed metabolic network that contains 102 metabolites and 133 reaction fluxes using the time profiles of citrate, dry cell, organic acids, amino acids, and SAP as the constraints. The model was solved by minimizing the SAP accumulation rate in the cell, The effects of the oxygen-tran...
Citation Formats
Z. Güldüren, “Causes and consequences of seasonal variation of phosphoglucomutase (pgm) enzyme polymorphism in honeybees, (apis mellifera l.) of turkey,” M.S. - Master of Science, Middle East Technical University, 2008.