Phenotypical characterization of microRNA-106B overexpression in MCF10A breast cell line

Download

index.pdf

Date

2013

Author

Saygılı, Cansaran

Metadata

Show full item record

Item Usage Stats

222
views

84
downloads

MicroRNAs are small non-coding RNAs which regulate gene expression by binding to 3’UTR of their target mRNAs. Deregulated expression of microRNAs is detected in many pathologies including different types of cancers. miR-106b, is a member of miR-106b-25 cluster and overexpressed in many cancers including breast cancer. Based on miR-106b overexpression, we hypothesized that miR-106b may be an oncogene candidate. To explore miR-106b related phenotypes, we used an already miR-106b transfected model cell line system. Stably transfected MCF10A cells were investigated for alterations in cell growth, motility, migration and invasion. Our results showed that miR-106b overexpression caused increased growth motility and migration. On the other hand, based on matrigel invasion assay miR-106b expression caused a reduction in cell invasion. Further studies are needed to be performed to understand the precise role of miR-106b in breast cancer. Studies are underway to detect possible miR-106b targets that may help to explain these phenotypical alterations.

Subject Keywords

Breast, RNA, Gene expression., Phenotype.

URI

http://etd.lib.metu.edu.tr/upload/12615544/index.pdf
https://hdl.handle.net/11511/22445

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Characterization of microRNA-125b expression in MCF7 breast cancer cells by ATR-FTIR spectroscopy OZEK, Nihal Simsek; TUNA, Serkan; Erson Bensan, Ayşe Elif; Severcan, FERİDE (2010-01-01) MicroRNAs (miRNAs), are similar to 22 nucleotides long, non-coding RNAs that control gene expression post-transcriptionally by binding to their target mRNA's 3'UTRs (untranslated regions). Due to their roles in various important regulatory processes and pathways, miRNAs have been implicated in disease mechanisms such as tumorigenesis when their expression is deregulated. To date, a significant number of miRNAs and their target messenger RNAs (mRNAs) have been identified and verified. It is generally accepte...
Characterization of molecular-level changes due to MicroRNA-125B expression in breast cancer cells by spectroscopic and chemometric analysis techniques Şimşek Özek, Nihal; Severcan, Feride; Erson Bensan, Ayşe Elif; Department of Biology (2015) MicroRNAs (miRNAs), are 22 nucleotides long, non-coding RNAs that control gene expression post-transcriptionally by binding to their target mRNA’s 3’ UTRs (untranslated regions). Due to their crucial roles in various important regulatory processes and pathways, miRNAs have been implicated in disease mechanisms such as tumorigenesis, metastasis and drug resistance when their expression is deregulated. Moreover, the regulatory roles of these micromanagers have been demonstrated in the metabolism of cancer cel...
Modeling the combined effect of RNA-binding proteins and micrornas in post-transcriptional regulation HafezQorani, Saber; Aydın Son, Yeşim; Kazan, Hilal; Department of Medical Informatics (2015) Post-transcriptional regulation (PTR) controls the gene expression between transcription and translation. Regulation at this level is carried out by the interactions of trans-acting RNA-binding proteins (RBPs) and microRNAs (miRNAs) with cis-regulatory elements in mRNA. Majority of previous work have focused on the effect of a single factor independent of other co-factors bound to the same mRNA. However, recent studies have shown that RBPs and miRNAs can act in cooperation or competition with each other. In...
Analysis of motifs in microRNA-transcription factor gene regulatory networks Sürün, Bilge; Acar, Aybar Can; Purutçuoğlu Gazi, Vilda; Department of Bioinformatics (2014) MicroRNAs are small non-coding RNA molecules which contain 21-25 nucleotides, and function in post transcriptional regulation by inhibiting the translation of mRNA targets. miRNAs typically affect gene regulation by forming composite feed forward circuits (cFFCs) which also comprise a transcription factor (TF) and a target gene. By analyzing these cFFCs, the contribution of miRNAs in altering TF networks can be revealed. These contributions could either be the de-escalation of the target gene repertoire or ...
Expression and activity analyses of industrially important extracellular enzymes produced by a bacilysin knock-out mutant of Bacillus Subtilis Aytekin, Samet; Özcengiz, Gülay; Okay, Sezer; Department of Molecular Biology and Genetics (2018) Bacilysin, the smallest peptide antibiotic known to date, is produced non-ribosomally by Bacillus subtilis by the collective actions of seven proteins transcribed from bacABCDEF operon and bacG gene. Bacilysin is a two-amino acid peptide composed of L-alanine and a modified amino acid, L-anticapsin. Bacilysin biosynthesis was shown to be strongly regulated by quorum sensing through the actions of global regulator proteins including Spo0K, Spo0H, Spo0A, ComQ/ComX, ComP/ComA as well as several Phr proteins, O...

Citation Formats

C. Saygılı, “Phenotypical characterization of microRNA-106B overexpression in MCF10A breast cell line,” M.S. - Master of Science, Middle East Technical University, 2013.