Design and characterization of 5-l autoclavable batch-culture bioreactor /

Ünsal Beyge, Şeyma
Bioreactors are systems that use a biological agent to perform a chemical reaction. Developments on the field of biotechnology made production of even previously known non-renewable petro-chemical products to be produced by microorganism by use of bioreactors possible. Also developments on tissue engineering made it possible to produce organs by using bioreactors. Therefore, bioreactors become integral parts of future economy and technology. In this study prototyping of a small scale bioreactor was performed. Heat transfer system which can cause temperature change of minimum 0.2 °C/seconds was produced. Also an agitation system that can operate up to 1500 rpm without any contamination risk was developed and produced. For effective foam/level control a miniature multi-probe foam/level sensor with 2 mm sensitivity and 50 mm working range was developed and tested.


Fabrication and characterization of porous hydroxyapatite and biphasic calcium phosphate ceramic as bone substitutes
Koc, N; Timucin, M; Korkusuz, Feza (2004-01-01)
In the present study, the calcium phosphate powders (HAP, HAP-TCP) were produced by a precipitation technique involving the use of aqueous solutions of calcium nitrate and diammonium hydrogen phosphate. Preforms of calcium phosphate ceramics were produced by using a modified slip casting technique. Porosity in the cast and in the sintered ceramic was obtained through custom made PMMA beads similar to200-300 mum size. The green ceramic samples were prepared by suspending the apatite powder and PMMA beads in ...
Optimization of synthetic route to PNA-T-OH monomers
Peptide nucleic acids are synthetic molecules crafted to mimic natural nucleic acids, and thus, they are widely utilized in many chemical, and, biomedical applications. Although there exist many approaches to synthesize monomers to date, there is still room to improve these methodologies. With this motivation, we compared some widely utilized synthetic routes to obtain N-Boc-PNA-T-OH, and N-Fmoc-PNA-T-OH. Our results indicate that N-Bocethylenediamine is the most pivotal intermediate in the chemistry of PNA...
Preparation and characterization of surface enhanced raman scattering substrate through electro deposition of silver-pedot film on ito glass surface
Doğan, Üzeyir; Volkan, Mürvet; Department of Chemistry (2011)
Detection of chemicals is a vital part of chemistry. For this reason, many detection systems are developed by scientists and every detection system has its own advantages. Raman spectroscopy is one of these detection systems having many advantages. However, this technique suffers from low signal intensity disadvantage. By developing a well prepared substrate, this problem can be easily solved; moreover, even single molecule detection can be possible. In this study, a novel surface enhanced Raman scattering ...
Determination of volatile organic compounds in different microenvironments by multibed adsorption and short-path thermal desorption followed by gas chromatographic-mass spectrometric analysis
Oznur, Kuntasal; Deniz, Karman; Daniel, Wang; TUNCEL, SEMRA; Tuncel, Süleyman Gürdal (2005-12-16)
A multiphase assurance approach was developed for the accurate and precise determination of volatile organic compounds (VOCs) in different microenvironments. This approach includes (i) development of a method including adsorption of VOCs onto a multisorbent media followed by short-path thermal desorption (SPTD) pre-concentration and gas chromatography (GC) coupled to a mass spectrometry (MS) quantification, (ii) validation of the sampling and analytical method and (iii) validation of the data using a multid...
Identification of differentially expressed proteins in wheat after benzothiadiazole treatment
Gunel, Aslihan; Asbahi, Adnan; Ozgazi, Nese; Akkaya, Mahinur (2012-12-01)
The systemic acquired resistance (SAR) mechanism is stimulated by biological and chemical agents in response to pathogen infection as a part of the innate immunity response system of plants. The externally applied synthetic chemical, benzo-(1,2,3)-thiadiazole-7-carbothioic acid-S-methyl ester (BTH), is also known to induce a SAR response in plants. Studies identifying genes induced or suppressed by BTH are limited. Only few genes responding to BTH are determined. The focus of this preliminary study is to id...
Citation Formats
Ş. Ünsal Beyge, “Design and characterization of 5-l autoclavable batch-culture bioreactor /,” M.S. - Master of Science, Middle East Technical University, 2014.