Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Investigation of structural and functional properties of OMPG mutants by FTIR spectroscopy
Download
index.pdf
Date
2017
Author
Yılmaz, İrem
Metadata
Show full item record
Item Usage Stats
269
views
99
downloads
Cite This
The subject of the current study is a bacterial protein OmpG from Escherichia coli. OmpG-16S and OmpG-16SL are the mutants of OmpG that are genetically engineered for potential biotechnological applications. In this study, structural and functional similarities and/or differences of these mutants with respect to the wild type OmpG were revealed using IR spectroscopy. Also, a new spectrometer attachment enabling in situ 1H/2H exchange was developed. For the collection of mass amount of spectral data from each experiment, an automated data collection computer code was developed as well.
Subject Keywords
Membrane proteins.
,
Recombinant proteins.
,
Infrared spectroscopy.
,
Fourier transform infrared spectroscopy.
URI
http://etd.lib.metu.edu.tr/upload/12621415/index.pdf
https://hdl.handle.net/11511/26747
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Structural modelling and functional analysis of the engineered small heat-shock protein, tpv-hsp14.3 from thermoplasma volcanium
Sheraj, Ilir; Kocabıyık, Semra; Department of Biology (2014)
In this study, a small heat shock protein tpv-Hsp14.3 from a thermoacidophilic Archaeon, Thermoplasma volcanium was studied. sHSPs are low molecular weight proteins involved in different stress responses to protect the cellular proteome and enhance survival of the host organism. For structure-function analysis of the protein, both experimental and computational tools were used. Sequence alignments with closely-related sHSPs showed high sequence conservation at the core α-Crystalline domain and a V/I/L-X-V/I...
Development of amperometric cholinesterase biosensors based on a conducting polymer for detection of anti-dementia drugs and pesticides
Turan, Janset; Toppare, Levent Kamil; Özkan, Necati; Department of Polymer Science and Technology (2016)
Cholinesterase enzymes are considered as one of the most important enzymes in living organisms and they are targeted by many inhibitors. Cholinesterase inhibitors are potentially toxic however they also have certain applications as treatment of dementia and Alzheimer’s diseases. These inhibitors can bind the active side of the cholinesterase enzymes forming stable complexes. This inhibitory effect can be used to construct fast and sensitive detection system. Also, conducting polymers are known to be well su...
Pattern search in pathogenic bacterial proteins for localization and secretory systems
Özcan, Orhan; Özcengiz, Gülay; Can, Tolga; Department of Biotechnology (2015)
Computational prediction of bacterial protein localization (BPL) is a very useful tool which provides clues about protein function. For pathogenic proteins in particular, detection of their subcellular location and their secretory pathways have great implications for vaccine and drug design. Cell surface and/or secreted proteins of microbes can also be used as biomarkers for sensor applications. At present, there are numerous BPL prediction algorithms and programs available, however, most of them give false...
Current applications of exopolysaccharides from lactic acid bacteria in the development of food active edible packaging
Moradi, Mehran; Guimarães, Jonas T; Şahin, Serpil (Elsevier BV, 2021-08-01)
The lactic acid bacteria (LAB) are usually recognized as safe for consumption and comprise several genera with different technological and health-promoting potential for food applications, including probiotic characteristics. Their exopolysaccharides (EPS) have interesting film-forming properties and may be used to produce edible packaging, as a structural enhancer, a bioactive agent or probiotic carrier in edible films and coatings. In these days, there is a specific demand for food products with reduced a...
Biophysical characterization of proteins in solution and human fluids for cancer diagnosis applications using fourier transform infrared spectroscopy
Abbas, Sherif Abbas Mousa; Severcan, Feride; Severcan, Mete; Department of Biology (2016)
Proteins play very important roles in cells regulation and structure. Understanding of proteins structures greatly help in understanding of the mechanism of action of these proteins. The optical spectroscopic techniques such as Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy can be used to study proteins in its native environment without complicated sample preparations that is required for a high resolution technique such as x-ray. In order to calculate the protein secondary struc...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
İ. Yılmaz, “Investigation of structural and functional properties of OMPG mutants by FTIR spectroscopy,” M.S. - Master of Science, Middle East Technical University, 2017.