Investigation of structural and functional properties of OMPG mutants by FTIR spectroscopy

Download
2017
Yılmaz, İrem
The subject of the current study is a bacterial protein OmpG from Escherichia coli. OmpG-16S and OmpG-16SL are the mutants of OmpG that are genetically engineered for potential biotechnological applications. In this study, structural and functional similarities and/or differences of these mutants with respect to the wild type OmpG were revealed using IR spectroscopy. Also, a new spectrometer attachment enabling in situ 1H/2H exchange was developed. For the collection of mass amount of spectral data from each experiment, an automated data collection computer code was developed as well. 

Suggestions

Biophysical characterization of proteins in solution and human fluids for cancer diagnosis applications using fourier transform infrared spectroscopy
Abbas, Sherif Abbas Mousa; Severcan, Feride; Severcan, Mete; Department of Biology (2016)
Proteins play very important roles in cells regulation and structure. Understanding of proteins structures greatly help in understanding of the mechanism of action of these proteins. The optical spectroscopic techniques such as Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy can be used to study proteins in its native environment without complicated sample preparations that is required for a high resolution technique such as x-ray. In order to calculate the protein secondary struc...
Development of amperometric cholinesterase biosensors based on a conducting polymer for detection of anti-dementia drugs and pesticides
Turan, Janset; Toppare, Levent Kamil; Özkan, Necati; Department of Polymer Science and Technology (2016)
Cholinesterase enzymes are considered as one of the most important enzymes in living organisms and they are targeted by many inhibitors. Cholinesterase inhibitors are potentially toxic however they also have certain applications as treatment of dementia and Alzheimer’s diseases. These inhibitors can bind the active side of the cholinesterase enzymes forming stable complexes. This inhibitory effect can be used to construct fast and sensitive detection system. Also, conducting polymers are known to be well su...
Current applications of exopolysaccharides from lactic acid bacteria in the development of food active edible packaging
Moradi, Mehran; Guimarães, Jonas T; Şahin, Serpil (Elsevier BV, 2021-08-01)
The lactic acid bacteria (LAB) are usually recognized as safe for consumption and comprise several genera with different technological and health-promoting potential for food applications, including probiotic characteristics. Their exopolysaccharides (EPS) have interesting film-forming properties and may be used to produce edible packaging, as a structural enhancer, a bioactive agent or probiotic carrier in edible films and coatings. In these days, there is a specific demand for food products with reduced a...
Investigation of cytocidal effect of K5 type yeast killer protein on sensitive microbial cells
Sertkaya, Abdullah; İzgü, Kadri Fatih; Department of Biology (2005)
Some yeasts secrete polypeptide toxins, which are lethal to other sensitive yeast cells, gram-positive pathogenic bacteria and pathogenic fungi. Therefore these are designated as killer toxins. Killer toxins are suggested as potent antimicrobial agents especially for the protection of fermentation process against contaminating yeasts, biological control of undesirable yeasts in the preservation of foods. Moreover they are promising antimicrobial agents in the medical field; due to immune system suppressing ...
Biophysical characterization and diagnosis of obesity from adipose tissue by fourier transform infrared spectroscopy and imaging
Küçük Baloğlu, Fatma; Severcan, Feride; Department of Biology (2017)
Obesity is a heterogeneous disorder originating from the enlargement of visceral (VAT) and subcutaneous (SCAT) adipose tissue mass in the body and this process usually results in disturbed glucose and lipid metabolism. The first part of this study aimed to characterize and compare VAT and SCAT with regard to biomolecular content and also investigate transdifferentiation between white and brown adipocytes. Regarding this aim, Fourier transform infrared (FTIR) microspectroscopy and uncoupling protein 1 (UCP1)...
Citation Formats
İ. Yılmaz, “Investigation of structural and functional properties of OMPG mutants by FTIR spectroscopy,” M.S. - Master of Science, Middle East Technical University, 2017.