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Investigation of structural and functional properties of OMPG mutants by FTIR spectroscopy
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Date
2017
Author
Yılmaz, İrem
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The subject of the current study is a bacterial protein OmpG from Escherichia coli. OmpG-16S and OmpG-16SL are the mutants of OmpG that are genetically engineered for potential biotechnological applications. In this study, structural and functional similarities and/or differences of these mutants with respect to the wild type OmpG were revealed using IR spectroscopy. Also, a new spectrometer attachment enabling in situ 1H/2H exchange was developed. For the collection of mass amount of spectral data from each experiment, an automated data collection computer code was developed as well.
Subject Keywords
Membrane proteins.
,
Recombinant proteins.
,
Infrared spectroscopy.
,
Fourier transform infrared spectroscopy.
URI
http://etd.lib.metu.edu.tr/upload/12621415/index.pdf
https://hdl.handle.net/11511/26747
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Graduate School of Natural and Applied Sciences, Thesis
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İ. Yılmaz, “Investigation of structural and functional properties of OMPG mutants by FTIR spectroscopy,” M.S. - Master of Science, Middle East Technical University, 2017.