Serotonergic modulation of behavioural choice in drosophila melanogaster

Yurt, Pınar
The monoamine modulator, serotonin (5-HT) has previously been shown to be involved in sleep, feeding, learning and memory, and anxiety in fruit flies. In this study, we used a behavioural paradigm where the flies choose to respond to either to a looming visual stimulus or an appetitive tastant. Using the GAL4-UAS binary system, selectively we activated or inactivated serotonergic neurons in the fly brain to understand the mechanisms of competitive inhibition between appetitive/feeding and aversive/escape behavioural systems. Unlike the results of previous experiments that involved acute manipulations of serotonergic neurons, our results failed to yield a reduction of feeding when we inactivated the same serotonergic neurons chronically using Gal4-R50H05>UASKir2.1. Likewise, we failed to observe overfeeding when we activated the same serotonergic neurons chronically using Gal4-R50H05>UAS-NaChBac. This discrepancy can be accounted for the difference between acute and chronic manipulations. However, because the visual responsiveness increased upon chronic activation of the serotonergic neurons, we suggest that the serotonergic neurons of interest might be modulating sensory integration areas rather than hunger per se.


Neuroprotective Efficacy of the Peroxisome Proliferator-Activated Receptor-gamma Ligand in Chronic Cerebral Hypoperfusion
SAYAN ÖZAÇMAK, HALE; SAYAN, Hale; BARUT, FİGEN; Jakubowska-Dogru, Ewa (Bentham Science Publishers Ltd., 2011-08-01)
Chronic cerebral hypoperfusion can cause learning and memory impairment and neuronal damage resembling the effects observed in vascular dementia. PPAR-gamma agonists were shown to modulate inflammatory response and neuronal death following cerebral ischemia. The present study was designed to evaluate possible neuroprotective effects of rosiglitazone, a PPAR-gamma agonist, in rat model of chronic cerebral hypoperfusion. Cerebral hypoperfusion was induced by permanent bilateral occlusion of the common carotid...
A Plasmonic method for the determination of serotonin
Avcı, Begüm; Volkan, Mürvet; Department of Chemistry (2019)
Serotonin is an important neurotransmitter for regulating many cognitive and behavioral functions. Its abnormal levels have been related with various diseases hence the determination of the serotonin is very important. Sialic acid is mostly found in acidic glycan chains, glycolipids and glycoproteins and it is known to have a specific affinity for serotonin. In this study, determination of serotonin by using plasmonic property of gold nanoparticles was aimed. After synthesizing gold nanoparticles through si...
The effects of repeated antibiotic administration to juvenile BALB/c mice on the microbiota status and animal behavior at the adult age
Ceylani, Taha; Jakubowska-Dogru, Ewa; Gurbanov, Rafig; Teker, Hikmet Taner; Gözen, Ayşe Gül (2018-06-01)
Recent studies carried on germ -free (GF) animal models suggest that the gut microbiota (GM) may play a role in the regulation of anxiety, mood, and cognitive abilities such as memory and learning processes. Consistently, any treatment disturbing the gut microbiota, including the overuse of antibiotics, may influence the brain functions and impact behavior. In the present study, to address this issue, two wide-spectrum antibiotics (ampicillin and cefoperazone, 1 g/l) were repeatedly applied throughout a 6-w...
SRC family kinase inhibitors antagonize the toxicity of multiple serotypes of botulinum neurotoxin in human embryonic stem cell-derived motor neurons.
Kiriş, Erkan; Nuss, JE; Wanner, LM; Peyser, BD; Du, HT; Gomba, GY; Kota, KP; Panchal, RG; Gussio, R; Kane, CD; Tessarollo, L; Bavari, S (2015-05-01)
Botulinum neurotoxins (BoNTs), the causative agents of botulism, are potent inhibitors of neurotransmitter release from motor neurons. There are currently no drugs to treat BoNT intoxication after the onset of the disease symptoms. In this study, we explored how modulation of key host pathways affects the process of BoNT intoxication in human motor neurons, focusing on Src family kinase (SFK) signaling. Motor neurons derived from human embryonic stem (hES) cells were treated with a panel of SFK inhibitors a...
Effects of chronic diazepam treatment and withdrawal on avoidance behavior
Ayvasik, B; Zagli, U; Palaoglu, O; Soygur, H; Ayhan, IH (1999-06-01)
The present study was designed to evaluate the effects of chronic diazepam (DZ) treatment with low doses and diazepam withdrawal on the two-way shuttle-box avoidance performance in rats. After 24 rats were randomly assigned into three groups (I mg/kg DZ, 2 mg/kg DZ and control groups), all animals were trained to avoid from an electrical foot shock (0.8 mA) delivered through the grid floor by crossing to the other compartment of the box. Following the avoidance training, the first group received I mg/kg DZ ...
Citation Formats
P. Yurt, “Serotonergic modulation of behavioural choice in drosophila melanogaster,” M.S. - Master of Science, Middle East Technical University, 2017.