A Plasmonic method for the determination of serotonin

Download

index.pdf

Date

2019

Author

Avcı, Begüm

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

276
views

106
downloads

Serotonin is an important neurotransmitter for regulating many cognitive and behavioral functions. Its abnormal levels have been related with various diseases hence the determination of the serotonin is very important. Sialic acid is mostly found in acidic glycan chains, glycolipids and glycoproteins and it is known to have a specific affinity for serotonin. In this study, determination of serotonin by using plasmonic property of gold nanoparticles was aimed. After synthesizing gold nanoparticles through sialic acid reduction, the method was simply based on the red shift in absorbance peak of gold nanoparticles at 520 nm upon aggregation of gold nanoparticles which was induced by the reaction between serotonin and sialic acid. This shift was seen only for serotonin among serotonin, dopamine, l-cysteine, l-aspartic acid and l-glutamic acid and it has shown that the determination of serotonin can be performed in a simple and selective way by measuring the absorbance with an Ultraviolet-Visible (UV-VIS) spectrophotometer. The method successfully worked for serotonin concentration between 10 and 500 µM. To be able to work with serotonin levels in blood between 0.5 and 1.7 µM some modifications on gold nanoparticles were performed. Making selective side of sialic acid available for serotonin binding was aimed by attaching carboxyl group of sialic acid onto amine group containing gold nanoparticles so that an enhancement in aggregation can be achieved. The studies on binding sialic acid to gold nanoparticles from its nonselective side for serotonin are in progress.

Subject Keywords

Serotonin., Keywords: Gold Nanoparticles, Sialic Acid, Serotonin, Colorimetric Determination.

URI

http://etd.lib.metu.edu.tr/upload/12624021/index.pdf
https://hdl.handle.net/11511/44544

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

A signal transduction score flow algorithm for cyclic cellular pathway analysis, which combines transcriptome and ChIP-seq data Isik, Zerrin; Ersahin, Tulin; Atalay, Mehmet Volkan; AYKANAT, CEVDET; Atalay, Rengül (2012-01-01) Determination of cell signalling behaviour is crucial for understanding the physiological response to a specific stimulus or drug treatment. Current approaches for large-scale data analysis do not effectively incorporate critical topological information provided by the signalling network. We herein describe a novel model- and data-driven hybrid approach, or signal transduction score flow algorithm, which allows quantitative visualization of cyclic cell signalling pathways that lead to ultimate cell response...
An investigation of brain-to-brain coherence in the prefrontal cortex during joint sentence reading and joint fluid reasoning tasks Çakır, Murat Perit; Taşcı, Çağatay (null; 2018-03-24) Lack of an interactive context for joint action is an important limitation in social neuroscience studies. Portable brain imaging technologies such as functional near-infrared spectroscopy (fNIRS) have made it practical to simultaneously monitor the brain hemodynamics of two or more people engaged in social interaction. In this study, the modulation of inter-brain coherence is investigated in two different joint activity scenarios. In the first experiment, 22 dyads simultaneously read aloud matching and mis...
A biophysical network model displaying the role of basal ganglia pathways in action selection Yucelgen, Cem; Denizdurduran, Berat; Metin, Selin; Elibol, Rahmi; Şengör, Neslihan Serap (2012-10-25) Basal ganglia circuits are known to have role in a wide range of behaviour spanning from movement initiation to high order cognitive processes as reward related learning. Here, the intention is to have a biophysically realistic model of basal ganglia circuit for voluntary motor action selection. The ultimate aim is to provide a framework for models which could help comprehension of complex processes. To fulfill this aim a model capable of simulating direct, indirect and hyperdirect pathways with modified Ho...
Identifying the putative G protein coupled receptor/s candidates of cocaine and amphetamine regulated transcript (CART) peptide Kasap, Merve; Son, Çağdaş Devrim; Department of Biology (2014) Cocaine and amphetamine regulated transcript (CART) peptides are neurotransmitters and endocrine factors in the nervous system and periphery. CARTPT codes for a neuropeptide hormone with a number of biological roles which are important for the mammalian physiology such as controlling feeding behavior, drug reward, bone remodeling, sensory processing, neuroendocrine function, stress anxiety, cardiovascular function, gastrointestinal motility and development. In hypothalamus, behavioral and drug studies showe...
Dysregulation of hypothalamic modulation in olanzapine treated male rats Sezlev-Bilecen, Deniz; AK, MEHMET; Yanık, Tülin (Elsevier BV, 2016-11-03) The mechanism of weight gain through application of olanzapine, a serotonin and dopamine receptor antagonist has not been fully understood. Weight gain and food intake are under the control of various neurohormones; POMC (proopiomelanocortin), CART (cocaine and amphetamine regulated transcript), AgRP (Agouti-related peptide) and NPY (neuropeptide Y) that are majorly synthesized and secreted from the arcuate nucleus (ARC) of hypothalamus. In this study, the alteration of the ARC neurohormone levels in rats w...

Citation Formats

B. Avcı, “A Plasmonic method for the determination of serotonin,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Chemistry., Middle East Technical University, 2019.