Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Investigation of cellular mechanisms of Ser9Leu proopiomelanocortin (POMC) mutation in neuronal cells
Download
index.pdf
Date
2019
Author
Vural, Merve
Metadata
Show full item record
Item Usage Stats
286
views
103
downloads
Cite This
Pro-opiomelanocortin (POMC) is a precursor protein and synthesized in many different tissues of the body. It is proteolytically cleaved into different biologically active peptides which have different physiological functions based on the tissues they are found. Some of these peptides act in the brain to reduce food intake. The main regulator of the food intake and energy homeostasis is the arcuate nucleus (ARC) within the hypothalamus, and hypothalamic neurons expressing POMC have important roles in the regulation of body weight and energy homeostasis. These neurons are stimulated by the signals from extracellular matrix (ECM) to release the contents of mature POMC vesicles. The active peptides of POMC are released into the ECM and lead to anorexigenic response which suppress the food intake. Due to the important role of POMC in the regulation of body weight, many studies have focused on mutations in POMC of obese people. One of these mutations is Ser9Leu POMC found among children having early-onset obesity. It is located in the signal peptide of POMC which is removed after the co-translation of the protein in the normal state of the cell. In this study, the cellular mechanisms of the Ser9Leu POMC mutation were investigated. It was hypothesized that Ser9Leu POMC inhibits the translocation of POMC into the endoplasmic reticulum (ER) and leads to the accumulation of mutant proteins which may be secreted in the constitutive secretory pathway. Therefore, mouse neuroblastoma cells (N2a) were transfected with wild-type and Ser9Leu POMC DNAs, and secreted proteins from N2a cells were analyzed with the Western blot method using anti-ACTH (adrenocorticotropic hormone). Results revealed that the Ser9Leu POMC mutation did not cause a different effect on the cellular mechanism for its secretion from the neuronal cells.
Subject Keywords
Proopiomelanocortin.
,
Neural stem cells.
,
Neurons.
,
Obesity.
URI
http://etd.lib.metu.edu.tr/upload/12623020/index.pdf
https://hdl.handle.net/11511/27938
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Investigation of the effect of sodium butyrate on the regulation of cyclooxygenase-2 in colon cancer cell lines Caco-2 and HT-29
Ülgen, Doğukan Hazar; Banerjee, Sreeparna; Department of Biology (2015)
Sodium butyrate (NaBt) is a four-carbon short-chain fatty acid histone deacetylase inhibitor (HDACi) that is available in the colon through the commensal microbiota-mediated fermentation of dietary fibers. It is the main source of energy for colonocytes, and is regarded to have tumor suppressive effects, most prominently in colorectal cancer (CRC). Cyclooxygenase-2 (COX-2) is a gene important in the inflammatory response due to its ability to convert arachidonic acid to prostaglandins. Overexpression and ov...
Molecular cloning, characterization, and expression analysis of a gene encoding a Ran binding protein (RanBP) in Cucumis melo L.
Baloglu, Mehmet Cengiz; Zakharov, Florence Negre; Öktem, Hüseyin Avni; Yücel, Ayşe Meral (2011-01-01)
Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP w...
Investigation of a mechanistic link between 15-Lipoxygenase-1 (15-LOX-1) and Metastasis Associated Protein 1 (MTA1) in human colorectal carcinoma cell lines
ÇAĞATAY, SİBEL; Banerjee, Sreeparna (2013-09-01)
Background: Metastasis Associated Protein 1 (MTA1) is a member of the nuclear remodeling and histone deacetylase (NuRD) complex that is known to repress the expression of several tumor suppressor genes. 15-lipoxygenase-1 (15-LOX-1), a member of the inflammatory eicosanoid pathway, has been shown by us and others to have an anti-tumorigenic role in colon cancer. ALOX15 was recently shown to be repressed by the NuRD complex. Previously we have reported that ectopic expression of 15-LOX-1 in colon cancer cells...
INVESTIGATION OF PHYSICAL INTERACTION BETWEEN Gαi AND Gαs PROTEINS VIA FRET IN LIVE CELLS
Balkan, Seyda Tuğçe; Son, Çağdaş Devrim; Küçük Baloğlu, Fatma; Department of Biochemistry (2021-8-11)
GPCR’s are seven-transmembrane receptors that transmit external signals to the intracellular environment via secondary messenger systems through heterotrimeric G proteins. Heterotrimeric G proteins consist of α and β-γ subunits. Until recent years, scientists thought that GPCR signal transduction occurs between one GPCR and one heterotrimeric G protein; however, recently, it has been shown that GPCR’s can make oligomers. Oligomerization of GPCR allows cells to tune the intensity of the signal and respond ap...
Structural characterization of recombinant bovine Go alpha by spectroscopy and homology modeling
MEGA TİBER, PINAR; Orun, Oya; Nacar, Cevdet; Sezerman, Ugur Osman; Severcan, Feride; Severcan, Mete; Matagne, Andre; KAN, BEKİ (2011-01-01)
Go, a member of heterotrimeric guanine nucleotide-binding proteins, is the most abundant form of G protein in the central and peripheral nervous systems. Go alpha has a significant role in neuronal development and function but its signal transduction mechanism remains to be clarified.
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. Vural, “Investigation of cellular mechanisms of Ser9Leu proopiomelanocortin (POMC) mutation in neuronal cells,” M.S. - Master of Science, Middle East Technical University, 2019.