Estimation of phytoplankton biomass using HPLC pigment analysis in the southwestern Black Sea

Ediger, D.
Soydemir, N.
Kıdeyş, Ahmet Erkan
The phytoplankton population of the southwestern Black Sea in May 2001 was studied by taxonomic analysis using microscopic examination and by pigment analyses using high-performance liquid chromatography (HPLC). Pigment data, which identified phytoplankton assemblages dominated by dinoflagellates, diatoms and coccolithophores in May 2001, were compared to phytoplankton cell counts and biomass. There were significant (p < 0.002-0.01, r=0.56-0.67) relationships between the taxon-specific pigment concentrations and the taxon-specific cell numbers during this sampling period. The ratios of chlorophyll-a to the dominant accessory pigments calculated by multiple linear regressions were 1.2 (chlorophyll-a: peridinin) in dinoflagellates, 1.8 (chlorophyll-a: fucoxanthin) in diatoms, and 2.66 (chlorophyll-a: 19'-hex on oyloxyfucoxanthin) in coccolithophores. HPLC-determined chlorophyll-a biomass correlated well with the sum of the group-specific pigment biomass (p < 0.001, r(2) = 0.95). The phytoplankton assemblage as revealed by the microscopic and HPLC analyses was thus made up of common Black Sea groups showing that HPLC pigment analysis can be used to quantify phytoplankton assemblages in the Black Sea based on simple ratios.


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© 2022 Elsevier B.V.Marker pigments are used to determine taxonomic composition and biomass of microalgae in different oceanic regions. However, sometimes discrepancies are encountered between microscopy and marker pigment based approaches principally because of altering environmental factors influencing diversity of phytoplankton. In the present investigation, marker pigments from HPLC-CHEMTAX analysis concurrent with carbon biomass estimated by microscopy were investigated during 2015–2016 at weekly inter...
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Species composition, community structure and spatial distribution of net phytoplankton in the southern Black Sea have been studied. In the fraction over 55 mum, diatoms were found to be predominant in cell abundance and followed by dinoflagellates. Net phytoplankters were found to be more abundant in the southwestern sector, especially near the Bosporus and near Sakarya River mouth. MDS (Multi-Dimensional Scaling) analysis has projected four distinct patches along the coast, two of which appear to occupy sm...
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Citation Formats
D. Ediger, N. Soydemir, and A. E. Kıdeyş, “Estimation of phytoplankton biomass using HPLC pigment analysis in the southwestern Black Sea,” DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, pp. 1911–1922, 2006, Accessed: 00, 2020. [Online]. Available: