Estimation of phytoplankton biomass using HPLC pigment analysis in the southwestern Black Sea

Date

2006-01-01

Author

Ediger, D.
Soydemir, N.
Kıdeyş, Ahmet Erkan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

227
views

0
downloads

The phytoplankton population of the southwestern Black Sea in May 2001 was studied by taxonomic analysis using microscopic examination and by pigment analyses using high-performance liquid chromatography (HPLC). Pigment data, which identified phytoplankton assemblages dominated by dinoflagellates, diatoms and coccolithophores in May 2001, were compared to phytoplankton cell counts and biomass. There were significant (p < 0.002-0.01, r=0.56-0.67) relationships between the taxon-specific pigment concentrations and the taxon-specific cell numbers during this sampling period. The ratios of chlorophyll-a to the dominant accessory pigments calculated by multiple linear regressions were 1.2 (chlorophyll-a: peridinin) in dinoflagellates, 1.8 (chlorophyll-a: fucoxanthin) in diatoms, and 2.66 (chlorophyll-a: 19'-hex on oyloxyfucoxanthin) in coccolithophores. HPLC-determined chlorophyll-a biomass correlated well with the sum of the group-specific pigment biomass (p < 0.001, r(2) = 0.95). The phytoplankton assemblage as revealed by the microscopic and HPLC analyses was thus made up of common Black Sea groups showing that HPLC pigment analysis can be used to quantify phytoplankton assemblages in the Black Sea based on simple ratios.

Subject Keywords

Phytoplankton composition, Microscopy, Photosynthetic pigments, Chemotaxonomy, Black Sea

URI

https://hdl.handle.net/11511/29867

Journal

DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY

DOI

https://doi.org/10.1016/j.dsr2.2006.04.018

Collections

Graduate School of Marine Sciences, Article

Suggestions

OpenMETU
Core

Marker pigments and carbon biomass of phytoplankton on the northeastern Mediterranean Sea coast Konucu, Merve; Eker-Develi, Elif; Örek, Hasan; Başduvar, Şehmuz; Kıdeyş, Ahmet Erkan (2022-05-01) © 2022 Elsevier B.V.Marker pigments are used to determine taxonomic composition and biomass of microalgae in different oceanic regions. However, sometimes discrepancies are encountered between microscopy and marker pigment based approaches principally because of altering environmental factors influencing diversity of phytoplankton. In the present investigation, marker pigments from HPLC-CHEMTAX analysis concurrent with carbon biomass estimated by microscopy were investigated during 2015–2016 at weekly inter...
Modeling the influence of hydrodynamic processes on anchovy distribution and connectivity in the black sea Karadeniz'deki hamsi dağılımı ve bölgeler arası bağlantısı üzerine hidrodinamik proseslerin etkisinin modellenmesi Fach Salihoğlu, Bettina Andrea (2014-01-01) Dispersal mechanisms of Black Sea anchovy larvae (Engraulis encrasicolus ponticus) across the Black Sea were studied with an individual based anchovy larvae model embedded in a Lagrangian model using surface currents calculated from daily dynamic height topography maps of altimeter data during a period of three years (2001-2003). Particles representing anchovy eggs were released at different sites during June to August and their movement was tracked over time. Drifters were advected for 36 days, representin...
On the formation of net phytoplankton patches in the southern Black Sea during the spring Uysal, Zahit (2002-10-01) Species composition, community structure and spatial distribution of net phytoplankton in the southern Black Sea have been studied. In the fraction over 55 mum, diatoms were found to be predominant in cell abundance and followed by dinoflagellates. Net phytoplankters were found to be more abundant in the southwestern sector, especially near the Bosporus and near Sakarya River mouth. MDS (Multi-Dimensional Scaling) analysis has projected four distinct patches along the coast, two of which appear to occupy sm...
Weekly Variations in Phytoplankton Structure of a Harbour in Mersin Bay north eastern Mediterranean Eker Develi, Elif; Kıdeyş, Ahmet Erkan (2000-01-01) Weekly variations in the phytoplankton composition of a harbour in Mersin Bay were studied with two methods: filtration for the assessment of >55 μm phytoplankton from July 1995 to June 1997; and sedimentation for the assessment of all phytoplankton (both>55 μm and <55 μm) between 15 February and 25 May in 1996. With both sampling methods, a total of 175 phytoplankton species were identified. In the filtered samples, the total diatom abundance was much higher than that of dinoflagellates. The highest diatom...
Distribution of phytoplankton in the southern Black Sea in summer 1996, spring and autumn 1998 Eker-Develi, E; Kıdeyş, Ahmet Erkan (2003-03-01) The species composition, abundance, and biomass of micro- (>15 mum) and nano- (<15 mu m) phytoplankton were studied along the southern Black Sea during June-July 1996 and March-April and September 1998. A total of 150 species were identified, similar to 50% of them being dinoflagellates. The average total phytoplankton abundance changed from 77 x 10(3) Cells l(-1) in spring to 110 x 10(3) cells l(-1) in autumn and biomass from 250 mu g l(-1) in summer to 1370 mu g l(-1) in spring. Based on the extensive sam...

Citation Formats

D. Ediger, N. Soydemir, and A. E. Kıdeyş, “Estimation of phytoplankton biomass using HPLC pigment analysis in the southwestern Black Sea,” DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, pp. 1911–1922, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29867.