Evaluation of heterotrophic and mixotrophic cultivation of novel Micractinium sp ME05 on vinasse and its scale up for biodiesel production

Date

2018-03-01

Author

Engin, Iskin Kose
Çekmecelioğlu, Deniz
Yücel, Ayşe Meral
Öktem, Hüseyin Avni

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

224
views

0
downloads

Direct disposal of vinasse, a by-product of molasses fermentation plants, threatens environmental health. This study investigated the usage of vinasse as a nutrient source for the heterotrophic and mixotrophic cultivation of novel Micractinium sp. ME05. The 500-mL flask experiments resulted in higher biomass productivities under mixotrophic conditions (0.16 +/- 0.01 g L-1 day(1)) than the heterotrophic conditions (0.13 +/- 0.01 g L-1 day(1)). A 1.7-fold increase in biomass productivity was achieved by scaling up from 500-mL flasks (0.16 +/- 0.01 g L-1 day(1)) to 2-L flasks (0.27 +/- 0.019 g L-1 day(1)). The 5-L bioreactor experiments resulted in a biomass productivity of 0.32 +/- 0.2 g L-1 day(1) and lipid productivity of 3.4 +/- 0.20 g L-1 day(-1). This study demonstrated that Micractinium sp. ME05 can be cultivated with vinasse to produce large amounts of biomass. The FAME profile of mixotrophic Micractinium sp. ME05 cells was promising for further biodiesel production. This study highlights the feasibility of industrial by-product-vinasse as the nutrient source for biomass and lipid productions using the novel Micractinium sp. ME05 cells.

Subject Keywords

Mixotrophic, Micractinium sp, FAME, Vinasse, Heterotrophic

URI

https://hdl.handle.net/11511/30136

Journal

BIORESOURCE TECHNOLOGY

DOI

https://doi.org/10.1016/j.biortech.2017.12.023

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Optimization of windrow food waste composting to inactivate pathogenic microorganisms Çekmecelioğlu, Deniz; Graves, RE; Davitt, NH (2005-09-01) Composting is a popular means of treating organic wastes, and properly controlled composting can destroy the pathogenic microorganisms present in wastes for a human- and environment-friendly end product. Therefore, optimization of windrow composting of food waste, manure, and bulking agents was evaluated for maximum pathogen inactivation (Salmonella and E. coli O157:H7). Seasonal effects on reductions of Salmonella and E. coli O157:H7 according to the compost temperatures were studied (90 to 150 days). Feca...
Assessment of PCB contamination, the potential for in situ microbial dechlorination and natural attenuation in an urban watershed at the East Coast of the United States Kaya, Devrim; Sowers, Kevin R.; Demirtepe, Hale; Stiell, Brian; Baker, Joel E.; İmamoğlu, İpek; Kjellerup, Birthe (Elsevier BV, 2019-09-15) Sediment contamination is a major environmental issue in many urban watersheds and coastal areas due to the potential toxic effects of contaminants on biota and human health. Characterizing and delineating areas of sediment contamination and toxicity are important goals of coastal resource management in terms of ecological and economical perspectives. Core and surficial sediment samples were collected from an industrialized urban watershed at the East Coast of the United Stated and analyzed to evaluate the ...
Modeling natural attenuation of petroleum hydrocarbons (btex) in heterogeneous aquifers Uçankuş, Tuğba; Ünlü, Kahraman; Department of Environmental Engineering (2005) Natural Attenuation can be an effective cleanup option for remediation of Groundwater contamination by BTEX. One of the important aspects of the methodology that has been recognized recently is that mass removal rates, the most important parameters used to determine effectiveness of the methodology, is controlled by groundwater flow regime, which to a large extent controlled by aquifer heterogeneity. Considering this recognition, the primary objective of this research is to quantitatively describe the relat...
Investigation of sugar metabolism in rhizopus oryzae Büyükkileci, Ali Oğuz; Hamamcı, Haluk; Department of Biotechnology (2007) Rhizopus oryzae is a filamentous fungus, which can produce high amounts of L(+)-lactic acid and produces ethanol as the main by-product. In an effort to understand the pyruvate branch point of this organism, fermentations under different inoculum and glucose concentrations were carried out. At low inoculum size (1x103 spores ml-1), high amount of lactate (78 g l-1) was produced, whereas high ethanol concentration (37 g l-1) was obtained at high inoculum sizes (1x106 spores ml-1). Decreasing working volume i...
Investigation of activated sludge bioflocculation : influence of magnesium ions Turtin, İpek; Sanin, Faika Dilek; Department of Environmental Engineering (2005) Activated sludge systems are the most widely used biological wastewater treatment processes all over the world. The main working principles of an activated sludge system are the oxidation of biologically degradable wastes by microorganisms and the subsequent separation of the newly formed biomass from the treated effluent. Separation by settling is the most troublesome stage of an activated sludge process. A decrease in the efficiency of the separation of microbial biomass from the treated effluent causes a...

Citation Formats

I. K. Engin, D. Çekmecelioğlu, A. M. Yücel, and H. A. Öktem, “Evaluation of heterotrophic and mixotrophic cultivation of novel Micractinium sp ME05 on vinasse and its scale up for biodiesel production,” BIORESOURCE TECHNOLOGY, pp. 128–134, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30136.