Evaluation of heterotrophic and mixotrophic cultivation of novel Micractinium sp ME05 on vinasse and its scale up for biodiesel production

Direct disposal of vinasse, a by-product of molasses fermentation plants, threatens environmental health. This study investigated the usage of vinasse as a nutrient source for the heterotrophic and mixotrophic cultivation of novel Micractinium sp. ME05. The 500-mL flask experiments resulted in higher biomass productivities under mixotrophic conditions (0.16 +/- 0.01 g L-1 day(1)) than the heterotrophic conditions (0.13 +/- 0.01 g L-1 day(1)). A 1.7-fold increase in biomass productivity was achieved by scaling up from 500-mL flasks (0.16 +/- 0.01 g L-1 day(1)) to 2-L flasks (0.27 +/- 0.019 g L-1 day(1)). The 5-L bioreactor experiments resulted in a biomass productivity of 0.32 +/- 0.2 g L-1 day(1) and lipid productivity of 3.4 +/- 0.20 g L-1 day(-1). This study demonstrated that Micractinium sp. ME05 can be cultivated with vinasse to produce large amounts of biomass. The FAME profile of mixotrophic Micractinium sp. ME05 cells was promising for further biodiesel production. This study highlights the feasibility of industrial by-product-vinasse as the nutrient source for biomass and lipid productions using the novel Micractinium sp. ME05 cells.


Optimization of windrow food waste composting to inactivate pathogenic microorganisms
Çekmecelioğlu, Deniz; Graves, RE; Davitt, NH (2005-09-01)
Composting is a popular means of treating organic wastes, and properly controlled composting can destroy the pathogenic microorganisms present in wastes for a human- and environment-friendly end product. Therefore, optimization of windrow composting of food waste, manure, and bulking agents was evaluated for maximum pathogen inactivation (Salmonella and E. coli O157:H7). Seasonal effects on reductions of Salmonella and E. coli O157:H7 according to the compost temperatures were studied (90 to 150 days). Feca...
Assessment of PCB contamination, the potential for in situ microbial dechlorination and natural attenuation in an urban watershed at the East Coast of the United States
Kaya, Devrim; Sowers, Kevin R.; Demirtepe, Hale; Stiell, Brian; Baker, Joel E.; İmamoğlu, İpek; Kjellerup, Birthe (Elsevier BV, 2019-09-15)
Sediment contamination is a major environmental issue in many urban watersheds and coastal areas due to the potential toxic effects of contaminants on biota and human health. Characterizing and delineating areas of sediment contamination and toxicity are important goals of coastal resource management in terms of ecological and economical perspectives. Core and surficial sediment samples were collected from an industrialized urban watershed at the East Coast of the United Stated and analyzed to evaluate the ...
Evaluation of molasses and vinasse performance for lipid production from green microalgae
Engin, Işkın; Öktem, Hüseyin Avni; Çekmecelioğlu, Deniz; Department of Biotechnology (2017)
Microalgae are considered a valuable source of energy that could be an alternative to fossil fuels. Due to their high photosynthetic efficiency and high biomass production, microalgae have drawn attention for biodiesel production. However, cost of carbon source is a major challenge in biodiesel production from microalgae. For this reason, alternative carbon sources are being considered. In this study, heterotrophic growth conditions for improvement of biomass production from thermo-resistant Micractinium sp...
Modeling natural attenuation of petroleum hydrocarbons (btex) in heterogeneous aquifers
Uçankuş, Tuğba; Ünlü, Kahraman; Department of Environmental Engineering (2005)
Natural Attenuation can be an effective cleanup option for remediation of Groundwater contamination by BTEX. One of the important aspects of the methodology that has been recognized recently is that mass removal rates, the most important parameters used to determine effectiveness of the methodology, is controlled by groundwater flow regime, which to a large extent controlled by aquifer heterogeneity. Considering this recognition, the primary objective of this research is to quantitatively describe the relat...
Investigation of sugar metabolism in rhizopus oryzae
Büyükkileci, Ali Oğuz; Hamamcı, Haluk; Department of Biotechnology (2007)
Rhizopus oryzae is a filamentous fungus, which can produce high amounts of L(+)-lactic acid and produces ethanol as the main by-product. In an effort to understand the pyruvate branch point of this organism, fermentations under different inoculum and glucose concentrations were carried out. At low inoculum size (1x103 spores ml-1), high amount of lactate (78 g l-1) was produced, whereas high ethanol concentration (37 g l-1) was obtained at high inoculum sizes (1x106 spores ml-1). Decreasing working volume i...
Citation Formats
I. K. Engin, D. Çekmecelioğlu, A. M. Yücel, and H. A. Öktem, “Evaluation of heterotrophic and mixotrophic cultivation of novel Micractinium sp ME05 on vinasse and its scale up for biodiesel production,” BIORESOURCE TECHNOLOGY, pp. 128–134, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30136.